Comparative assessment of commercial enzyme-linked immunosorbent assay & rapid diagnostic tests used for dengue diagnosis in India

The Indian Journal of Medical Research
Ruta KulkarniVidya A Arankalle

Abstract

Dengue diagnosis is routinely carried out by detection of dengue virus (DENV) antigen NS1 and/or anti-DENV IgM antibodies using enzyme-linked immunosorbent assays (ELISAs) and rapid diagnostic tests (RDTs). This study was aimed at evaluation of quality of diagnostic assays currently in use in India for the identification of DENV infection. During 2016 dengue season (July-November) in Pune, India, comparative assessment of a few immunoassays was undertaken using (i) WHO-approved Panbio-Dengue-Early-(NS1)-ELISA and Panbio-Dengue-IgM-Capture-ELISA as reference tests, and (ii) Bayesian latent class analysis (BLCA) which assumes that no test is perfect. The assays included J.Mitra-Dengue-NS1-Ag-MICROLISA (JME-NS1), J.Mitra-Dengue-IgM-MICROLISA (JME-IgM), and two RDTs, namely, J.Mitra-Dengue-Day-1-Test (JM-RDT) and SD-BIOLINE-Dengue-Duo (SDB-RDT). Serum samples from patients seeking dengue diagnosis (n=809) were tested using the diagnostic kits. The presence of NS1 and/or IgM was taken as evidence for dengue-positive diagnosis. Panbio-NS1/IgM-ELISAs identified 38.6 per cent patients as dengue positive. With Panbio-ELISA as reference, all the tests were less sensitive for IgM detection, while for NS1, JM-RDT was less sensitive. For co...Continue Reading

References

Oct 7, 2004·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Sumalee ChanamaPathom Sawanpanyalert
Apr 1, 2006·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Stuart D BlacksellNicholas P J Day
Aug 24, 2007·Clinical and Vaccine Immunology : CVI·Stuart D BlacksellPaul N Newton
Feb 26, 2009·Emerging Infectious Diseases·Elizabeth A HunspergerRosanna W Peeling
Apr 23, 2010·Clinical and Vaccine Immunology : CVI·Kovi BessoffElizabeth Hunsperger
Apr 30, 2010·Vector Borne and Zoonotic Diseases·Kwoon-Yong PokLee-Ching Ng
Jul 14, 2010·PLoS Neglected Tropical Diseases·Monique da Rocha Queiroz LimaFlavia Barreto dos Santos
Feb 10, 2011·Indian Journal of Medical Microbiology·A ShrivastavaP V Lakshmana Rao
May 7, 2011·Nature Reviews. Microbiology·Rosanna W PeelingSutee Yoksan
Mar 24, 2012·Clinical and Vaccine Immunology : CVI·Stuart D BlacksellDavid G Lalloo
Apr 9, 2013·Nature·Samir BhattSimon I Hay
Mar 18, 2016·The Journal of Infectious Diseases·Elizabeth A HunspergerHarold S Margolis
May 18, 2016·The American Journal of Tropical Medicine and Hygiene·Leidy D PiedrahitaBerta N Restrepo
May 27, 2016·Journal of Clinical Microbiology·Elizabeth A HunspergerHarold S Margolis
Dec 14, 2016·Diagnostic Microbiology and Infectious Disease·Rosario VivekAnita Shet
Aug 24, 2017·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·C SimonnetA Mahamat

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
ELISAs
ELISA

Software Mentioned

JME
RStudio
JM
RDT
NS1

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