Comparative assessment of various lipid extraction protocols and optimization of transesterification process for microalgal biodiesel production

Environmental Technology
Shovon MandalNirupama Mallick

Abstract

Biodiesel, using microalgae as feedstocks, is being explored as the most potent form of alternative diesel fuel for sustainable economic development. A comparative assessment of various protocols for microalgal lipid extraction was carried out using five green algae, six blue-green algae and two diatom species treated with different single and binary solvents both at room temperature and using a soxhlet. Lipid recovery was maximum with chloroform-methanol in the soxhlet extractor. Pretreatments ofbiomass, such as sonication, homogenization, bead-beating, lyophilization, autoclaving, microwave treatment and osmotic shock did not register any significant rise in lipid recovery. As lipid recovery using chloroform-methanol at room temperature demonstrated a marginally lower value than that obtained under the soxhlet extractor, on economical point of view, the former is recommended for microalgal total lipid extraction. Transesterification process enhances the quality of biodiesel. Experiments were designed to determine the effects of catalyst type and quantity, methanol to oil ratio, reaction temperature and time on the transesterification process using response surface methodology. Fatty acid methyl ester yield reached up to 91% w...Continue Reading

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Citations

Jul 23, 2016·Frontiers in Microbiology·Nirupama MallickAkhilesh K Singh
Dec 7, 2018·Environmental Science and Pollution Research International·Anbarasan Tamilalagan, Jayanthi Singaram
Mar 4, 2021·Critical Reviews in Biotechnology·Bermejo ElisabethTaidi Behnam

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