Comparative binding of FSH to chicken and rat testis

Journal of Endocrinological Investigation
W L GordonD N Ward

Abstract

The binding characteristics of two highly purified preparations of follicle-stimulating hormone (FSH), one from equine (e) and one from porcine (p), were compared in a chicken testis radioreceptor assay. A two-component binding model was adequate to explain the binding of eFSH or pFSH to the chicken testis homogenate (CTH) FSH binding sites in the Scatchard study. The affinity of eFSH for the FSH binding site of CTH was 10-fold greater than that of pFSH by Scatchard analysis. This is consistent with the observation that in competitive protein binding studies approximately 10-fold greater quantities of pFSH were required to displace [125I] eFSH from the CTH FSH binding site than eFSH. Qualitatively, the order of relative potencies for various gonadotropin preparations was the same in either the CTH or a rat testis receptor radioligand assay. However, quantitatively, higher potency estimates were obtained with the CTH. The ability of LH preparations to displace radioiodinated pFSH or eFSH from CTH was generally very low with the notable exception of equine LH. The relative potency of highly purified eLH was approximately equal to that of the NIH-FSH-S16 standard (0.906 x NIH-FSH-S16) indicating that in the chicken, as in the rat,...Continue Reading

References

Sep 1, 1978·The American Journal of Physiology·K M BaldwinR E Lewis
May 1, 1974·General and Comparative Endocrinology·L E Reichert, V K Bhalla
Dec 1, 1973·European Journal of Clinical Investigation·S SchwartzD Rabinowitz
Sep 1, 1980·Analytical Biochemistry·P J Munson, D Rodbard

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Citations

Aug 15, 2001·The Journal of Clinical Endocrinology and Metabolism·W J WaltonG R Bousfield
Apr 29, 2020·Journal of the Endocrine Society·Teresa ZariñánAlfredo Ulloa-Aguirre
Jan 17, 2008·Biochemistry·George R BousfieldHeather Desaire
Dec 20, 1996·Molecular and Cellular Endocrinology·G R BousfieldW T Moore
Nov 3, 2006·Molecular and Cellular Endocrinology·George R BousfieldNaomi E Rance
Jun 9, 2009·Animal Reproduction Science·María Victoria FachalP Jorge Chedrese

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