Comparative characterization of hair follicle dermal stem cells and bone marrow mesenchymal stem cells

Stem Cells and Development
Martin J HoogduijnPaul G Genever

Abstract

We compared the growth and differentiation characteristics of hair follicle-derived dermal stem cells with bone marrow mesenchymal stem cells (MSCs). Follicular dermal cells were isolated from whisker hairs of Wistar rats and bone marrow MSCs were isolated from femora of the same animals. The adherent hair follicle dermal cells showed a fibroblastic morphology in serum-containing culture medium, were CD44(+), CD73(+), CD90(+), and CD34(), and had a population doubling time of 27 h. MSCs isolated from the bone marrow showed a similar morphology and population doubling time and expressed the same cell-surface markers. Following exposure to appropriate induction stimuli, both cell populations had the capacity to differentiate into various mesenchymal lineages, such as osteoblasts, adipocytes, chondrocytes, and myocytes and expressed neuroprogenitor cell markers. The rate and extent of differentiation were remarkably similar for both hair follicleand bone marrow-derived cells, whereas interfollicular dermal cells failed to differentiate. We identified telomerase activity in follicle dermal stem cells and marrow MSCs and demonstrated that they were capable of clonal expansion. In ex vivo analyses, we identified the presence of putat...Continue Reading

References

Jan 1, 1988·Journal of Cell Science. Supplement·F M Watt
Jan 1, 1988·Journal of Cell Science. Supplement·C S Potten, R J Morris
Mar 13, 1999·The Journal of Clinical Investigation·S MakinoS Ogawa
Jun 10, 2000·British Journal of Haematology·A EricesJ J Minguell
Mar 30, 2001·Bone Marrow Transplantation·O N Koç, H M Lazarus
Sep 5, 2001·Nature Cell Biology·J G TomaF D Miller
Mar 26, 2002·Journal of Dermatological Science·Fiona M Watt
Jun 27, 2002·Proceedings of the National Academy of Sciences of the United States of America·Edwin M HorwitzTed Hofmann
Sep 24, 2002·Journal of Cell Science·Majlinda LakoColin A B Jahoda
Dec 12, 2002·Molecular Biology of the Cell·Patricia A ZukMarc H Hedrick
Apr 19, 2003·Experimental Dermatology·A GharziC A B Jahoda
May 24, 2003·Leukemia·S ZimmermannU M Martens
Jun 6, 2003·The Journal of Investigative Dermatology·Desmond J TobinRalf Paus
Jul 2, 2003·Cells, Tissues, Organs·Daniel A De UgarteMarc H Hedrick
Aug 5, 2003·The Journal of Investigative Dermatology. Symposium Proceedings·Robert M LavkerAngela M Christiano
Aug 9, 2003·Proceedings of the National Academy of Sciences of the United States of America·Lingna LiRobert M Hoffman
Dec 6, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Pieter de LangeAntonia Lanni
Jan 13, 2004·Experimental Dermatology·Colin A B JahodaNicholas Hole
Jul 1, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·James P Maloney
Nov 2, 2004·Nature Cell Biology·Karl J L FernandesFreda D Miller
Nov 24, 2004·Differentiation; Research in Biological Diversity·Tatiana TondreauDominique Bron
Jun 1, 2005·Laboratory Investigation; a Journal of Technical Methods and Pathology·Federica SabatiniDanièle Brouty-Boyé

❮ Previous
Next ❯

Citations

Mar 12, 2010·Der Hautarzt; Zeitschrift für Dermatologie, Venerologie, und verwandte Gebiete·K Sellheyer, D Krahl
Aug 14, 2012·Cell and Tissue Research·Radhakrishnan VishnubalajiThiyagarajan Ramesh
Aug 1, 2008·Chinese Journal of Traumatology = Zhonghua Chuang Shang Za Zhi·Cui-ping Zhang, Xiao-bing Fu
Aug 5, 2008·Stem Cells and Development·Gang WuYan Jin
Jan 23, 2013·Stem Cells and Development·Joong Hyun ShimDong Wook Shin
Dec 17, 2009·Tissue Engineering. Part a·Hsing-I HuangShu-Hui Chan
Mar 20, 2010·Tissue Engineering. Part a·Jin Yu LiuStelios T Andreadis
May 11, 2012·Tissue Engineering. Part B, Reviews·Vivek K Bajpai, Stelios T Andreadis
Nov 20, 2012·Tissue Engineering. Part B, Reviews·Panagiotis Mistriotis, Stelios T Andreadis
Mar 5, 2008·Cardiovascular Research·Jin Yu LiuStelios T Andreadis
Sep 18, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Raja K SivamaniR Rivkah Isseroff
Sep 4, 2008·Plastic and Reconstructive Surgery·Harvey ChimArun K Gosain
Sep 11, 2009·Transplantation·Marc H DahlkeUNKNOWN MISOT Study Group
Feb 7, 2007·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Karl J L FernandesFreda D Miller
Nov 28, 2007·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Kuniko KadoyaWilliam B Stallcup
Feb 12, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Nathaniel A SowaMark J Zylka
Nov 17, 2009·Expert Review of Medical Devices·Thomas HodgkinsonArdeshir Bayat
Nov 13, 2015·Expert Opinion on Biological Therapy·Lunchang WangBo Yang
Jul 3, 2010·Biochemical and Biophysical Research Communications·Ji Hoon YangHoon Taek Lee
May 18, 2010·Journal of the American Academy of Dermatology·Klaus Sellheyer, Dieter Krahl
Oct 20, 2009·European Journal of Pharmacology·Zbigniew DarzynkiewiczDonald Wlodkowic
Nov 4, 2011·The Journal of Investigative Dermatology·Christine VaculikAdelheid Elbe-Bürger
Sep 24, 2008·Experimental Gerontology·Christos C ZouboulisCatherin Niemann
Nov 22, 2007·Biochimie·Nedime SerakinciSteen Kolvraa
Sep 18, 2007·Burns : Journal of the International Society for Burn Injuries·Juergen SchlabeMarkus V Küntscher
Sep 21, 2007·European Journal of Cell Biology·Jennifer KajahnSandra Danner
Jun 20, 2007·European Journal of Cell Biology·Stephan TiedeRalf Paus
May 8, 2007·Seminars in Cell & Developmental Biology·James M WatersColin A B Jahoda
Sep 2, 2009·Experimental Dermatology·Katherine LauArdeshir Bayat
Jan 24, 2007·Oral Diseases·P Stephens, P Genever
Apr 26, 2013·Experimental Dermatology·Kiarash Khosrotehrani
Mar 2, 2011·Experimental Dermatology·Angela Ariza de SchellenbergerGerd Lindner
Dec 14, 2011·Experimental Dermatology·Haruyo YamanishiTsutomu Soma
Dec 22, 2010·International Journal of Dermatology·Enas A S AttiaSaleh M El-Shiemy
Feb 16, 2011·Journal of Cellular Biochemistry·Carola CavalloLorenzo Moroni
Mar 13, 2014·Biological Reviews of the Cambridge Philosophical Society·Joseph N Nissimov, Asit Baran Das Chaudhuri

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.