Comparative characterization of two tests for measurement of hepatitis B virus DNA in the blood serum and plasma, based on the use of two different detection methods

Bulletin of Experimental Biology and Medicine
A Juman AwadhM I Mikhailov

Abstract

Two tests for measurements of hepatitis B virus DNA in human serum and plasma, VERSANT HBV 3.0 (based on the use of branched DNA chains) and Biotitre-B (a real-time PCR variant), were compared. Serum specimens from patients (n=56) with documented viral hepatitis were tested. For specimens with DNA values in the linear range in both tests the correlations were evaluated by Pearson's method. The sensitivity of the two tests, and reproducibility of the results of HBV DNA measurements were evaluated using a panel of recombinant virus DNA dilutions with a step of 1 lg HBV DNA copies per ml (from 7 to 3 lg). Three measurements for each concentration of the reference sample were carried out in each test. Clinical specificity of the two tests was evaluated by the analysis of HBV-negative serum samples, collected from donors not reactive by HBsAg and anti-HBc (n=60). Of 56 samples with documented infection, the results of testing by VERSANT HBV 3.0 and Biotitre-B did not agree in 4 (7.1%) samples. Pearson's correlation coefficient of for results obtained in VERSANT HBV 3.0 and Biotitre-B in linear range for both tests was 0.712. Evaluation of reproducibility of the tests using a panel of recombinant HBV DNA showed higher reproducibility...Continue Reading

References

Nov 26, 2002·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Chi-Jen ChuAnna S F Lok
Jan 16, 2004·Scandinavian Journal of Infectious Diseases·Ann SöderströmMagnus Lindh
Mar 5, 2004·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Anna S F LokUNKNOWN Practice Guidelines Committee, American Association for the Study of Liver Diseases (AASLD)
Mar 16, 2004·Clinical Gastroenterology and Hepatology : the Official Clinical Practice Journal of the American Gastroenterological Association·Emmet B KeeffeTeresa L Wright
Oct 16, 2004·Clinics in Liver Disease·W Graham Cooksley
Jan 5, 2006·JAMA : the Journal of the American Medical Association·Chien-Jen ChenUNKNOWN REVEAL-HBV Study Group

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Citations

Dec 30, 2014·Orvosi hetilap·Péter HollóSarolta Kárpáti
Aug 12, 2014·World Journal of Gastroenterology : WJG·Filippo AnsaldiGiancarlo Icardi
Oct 21, 2015·World Journal of Hepatology·Mallika GhoshMalay Kumar Saha

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