Comparative Characterization of Vaginal Cells Derived From Premenopausal Women With and Without Severe Pelvic Organ Prolapse

Reproductive Sciences
Hala KufaishiOksana Shynlova

Abstract

This study tested a hypothesis that primary human vaginal cells derived from tissue of premenopausal women with severe pelvic organ prolapse (POP-HVCs) would display differential functional characteristics as compared to vaginal cells derived from asymptomatic women with normal pelvic floor support (control-HVCs). Vaginal tissue biopsies were collected from premenopausal patients with POP (n = 8) and asymptomatic controls (n = 7) during vaginal hysterectomy or repair. Primary vaginal cells were isolated by enzymatic digestion and characterized by immunocytochemistry. Cell attachment and proliferation on different matrices (collagen I, collagen II, collagen IV, fibronectin, laminin, tenascin, and vitronectin) were compared between POP-HVCs and control-HVCs. RNA was extracted, and the expression of 84 genes was screened using Human Extracellular Matrix and Adhesion Molecules RT(2) Profiler PCR array. The expression of selected genes was verified by quantitative reverse transcription-polymerase chain reaction. (1) Control-HVCs attached to collagen IV more efficiently than POP-HVCs; (2) control-HVCs and POP-HVCs show a similar proliferation rate when plated on proNectin and collagen I; (3) when seeded on collagen I, resting POP-HVC...Continue Reading

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Citations

Sep 22, 2020·Journal of Immunology Research·Radu Dragos MarcuOvidiu Gabriel Bratu
Apr 8, 2021·Reproductive Sciences·Jennifer M McCrackenJulie C E Hakim
Aug 5, 2021·Experimental and Therapeutic Medicine·Zhi-Min DengYan-Xiang Cheng

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Methods Mentioned

BETA
biopsies
hysterectomy
biopsy
dissection
Profiler
PCR

Software Mentioned

Primer
BLAST
CFX Manager
Graph Pad
Prism

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