Comparative cytotoxicity of the aqueous chlorination products of thiobencarb, a thiocarbamate herbicide, in cultured rat hepatocytes

Toxicology in Vitro : an International Journal Published in Association with BIBRA
H JinnoM Ando

Abstract

Thiobencarb (S-4-chlorobenzyl N, N-diethylthiocarbamate) has been widely used in the rice fields of Japan. This herbicide is reported to decompose in aqueous chlorination to the compounds 4-chlorotoluene, 4-chlorobenzyl chloride, 4-chlorobenzyl alcohol, 4-chlorobenzaldehyde and 4-chlorobenzoic acid. We compared their cytotoxicity and the inducibility of cytochrome P-450 (P450) in cultured rat hepatocytes. Of the six compounds including thiobencarb, 4-chlorobenzyl chloride was the most hepatotoxic (EC(50): 0.17 mm), followed by thiobencarb (0.69 mm) and 4-chlorotoluene (1.2 mm). 4-Chlorobenzyl alcohol (4.6 mm) and 4-chlorobenzaldehyde (4.6 mm) were less toxic than thiobencarb, and 4-chlorobenzoic acid was the least toxic ( > 6.0 mm). From the results of the TBARS (2-thiobarbituric acid reactive substance) assay, lipid peroxidation was shown to be involved in the hepatotoxicity of 4-chlorobenzyl chloride, and less probably in that of thiobencarb and 4-chlorobenzaldehyde. 4-Chlorobenzoic acid and 4-chlorobenzaldehyde induced hepatic ethoxyresorufin O-deethylase and pentoxyresorufin O-depentylase activities, respectively. The induction of enzyme activities was accompanied by the increase in the corresponding P-450 apoprotein. Furth...Continue Reading

References

Jan 1, 1976·Methods in Cell Biology·P O Seglen
Nov 15, 1976·Experientia·D A Young
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jun 1, 1991·The Biochemical Journal·J P Landers, N J Bunce
Jan 1, 1991·Methods in Enzymology·D LiP S Guzelian
Nov 1, 1991·The Tohoku Journal of Experimental Medicine·N InomataM Yamamoto
Sep 1, 1989·Archives of Environmental Contamination and Toxicology·S Saxena, M S Abdel-Rahman
May 1, 1988·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·H O JaureguiS Naik
Nov 1, 1987·Chemistry and Physics of Lipids·G PoliM U Dianzani
Mar 1, 1988·Journal of Cellular Physiology·E G SchuetzP S Guzelian
Jan 1, 1987·Free Radical Biology & Medicine·C G FragaA L Tappel
Jun 1, 1982·Biochemical Pharmacology·C R WolfC M Schiller
Feb 1, 1995·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·N HaniokaM Ando
Oct 1, 1993·Critical Reviews in Oncology/hematology·M U Dianzani
Jul 6, 1993·Biochemical Pharmacology·C J MattiaS C Bondy

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.