PMID: 9432039Jan 31, 1998Paper

Comparative detection and quantitation of human CDK inhibitor mRNA expression of p15INK4B, p16INK4A, p16beta, p18INK4C, p19INK4D, p21WAF1, p27KIP1 and p57KIP2 by RT-PCR using a polycompetitive internal standard

British Journal of Haematology
J SchwallerA Tobler

Abstract

For comparative and quantitative analysis of human cyclin-dependent kinase inhibitor gene expression (CKI; p15INK4B, p16INK4A, p16beta, p18INK4C, p19INK4D, p21WAF1, p27KIP1 and p57KIP2) we set up an RT-PCR assay with a construct termed pCKIquant producing polycompetitive RNA as an internal standard. We demonstrated the reproducibility, accuracy and high sensitivity of the assay in the in vitro model of myeloid leukaemic HL-60 cells. We also showed that the pCKIquant CKI assay is an excellent tool for the assessment of CKI mRNA expression in clinical samples, e.g. single cryostat sections of lymphoma biopsies.

Citations

Jun 1, 2005·Biochemical and Biophysical Research Communications·Adam GeminGurmit Singh
Apr 13, 2001·Experimental Hematology·J R Fink, T W LeBien
Jul 13, 2004·The Journal of Investigative Dermatology·Naoko Kanda, Shinichi Watanabe

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