Comparative diagnostics of allergy using quantitative immuno-PCR and ELISA

Bioanalysis
Maria A SimonovaElena V Svirshchevskaya

Abstract

Estimation of specific IgE is essential for the prevention of allergy progression. Quantitative immuno-PCR (qiPCR) can increase the sensitivity of IgE detection. We aimed to develop qiPCR and compare it to the conventional ELISA in identification of IgE to Alt a 1 and Fel d 1 allergens. Single stranded 60-mer DNA conjugated to streptavidin was used to detect antigen-IgE-biotin complex by qiPCR. In semi-logarithmic scale qiPCR data were linear in a full range of serum dilutions resulting in three- to ten-times higher sensitivity of qiPCR in comparison with ELISA in IgE estimation in low titer sera. Higher sensitivity of qiPCR in identification of low titer IgE is a result of a higher linearity of qiPCR data.

References

Nov 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·J P MorgensternM C Kuo
Jan 11, 2013·Clinical and Vaccine Immunology : CVI·Micah D HalpernMollie W Jewett
Apr 22, 2015·Bioorganicheskaia khimiia·A V MaerleS K Zavriev
Oct 1, 2015·Diagnostic Microbiology and Infectious Disease·Netrapal SinghPromod K Mehta
Feb 4, 2016·Materials Science & Engineering. C, Materials for Biological Applications·Liu Chia-ChingLin Feng-Huei
Feb 14, 2016·Analytica Chimica Acta·Le ChangLunan Wang
Jul 30, 2016·Clinical Immunology : the Official Journal of the Clinical Immunology Society·E SvirshchevskayaS Zavriev
Oct 11, 2016·Molecular and Cellular Probes·Samine RahmatpourEsmaeil Sadroddiny
Jan 17, 2017·Glycoconjugate Journal·Artem V MaerleDmitriy Yu Ryazantsev
Mar 7, 2017·Biochemistry. Biokhimii︠a︡·D Y RyazantsevS K Zavriev
Dec 13, 2017·Scandinavian Journal of Clinical and Laboratory Investigation·Anna Sergeevna DolgovaOlga Alekseevna Stukolova

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
ELISA
surface plasmon resonance
FCS

Software Mentioned

RIDA
qiPCR

Related Concepts

Related Feeds

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.