Comparative effects of autologous and homologous seminal plasma on the viability of largely extended boar spermatozoa

Reproduction in Domestic Animals = Zuchthygiene
Ignacio CaballeroE A Martinez

Abstract

Sperm handling, associated to artificial reproduction technologies (ART) such as in vitro fertilization (IVF) or the use of flow cytometry for cell analysis or sorting imposes volumetric extension of the sperm suspension and decreases sperm viability, presumably because of the removal of seminal plasma (SP) components. This study evaluated whether a 10% v/v of autologous SP (retrieved from the same donor boar) or homologous SP (e.g. from any of the four fertile boars included, other than the one providing the spermatozoa) would differently affect the viability of boar spermatozoa subjected to large extension in a simple saline medium [phosphate-buffered saline and 0.1% ethylenediaminetetraacetic acid (EDTA), PBSm] to a concentration of 0.3 x 10(6) spermatozoa/ml and incubated for 2 h at 30 degrees C. Sperm viability was monitored as membrane integrity [using the fluorophore carboxyfluorescein diacetate (C-FDA) and propidium iodide (PI)], mitochondrial function (using the fluorophore R-123) and motility characteristics [using Computer Assisted Sperm Analysis (CASA)]. Substraction of the SP and extension followed by incubation in PBSm significantly (p < 0.05) decreased sperm viability, which could be restored by addition of autol...Continue Reading

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Citations

Jun 16, 2012·Tropical Animal Health and Production·María Bottini-LuzardoJosé Herrera-Camacho
Apr 23, 2009·Reproduction, Fertility, and Development·T LeahyW M C Maxwell
May 3, 2011·Polish Journal of Veterinary Sciences·A Dziekońska, J Strzezek
Jan 24, 2012·Animal Reproduction Science·José Gómez-FernándezEduardo de Mercado
Jan 12, 2010·Animal Reproduction Science·T LeahyW M C Maxwell
Dec 1, 2009·Animal Reproduction Science·J C GarciaR N Kirkwood
Nov 4, 2008·Theriogenology·J M VazquezE A Martínez
Sep 3, 2008·Theriogenology·S P de GraafW M C Maxwell
Aug 5, 2008·Theriogenology·J M VazquezE A Martínez
Jul 22, 2008·Theriogenology·H Rodríguez-MartínezF J Peña
Jul 31, 2007·Theriogenology·E M GarcíaE A Martínez
Apr 11, 2008·International Journal of Andrology·Ignacio CaballeroEmilio A Martinez
Jul 25, 2008·Reproduction in Domestic Animals = Zuchthygiene·D Rath, L A Johnson
Jul 27, 2007·International Journal of Andrology·E M GarcíaH Rodríguez-Martínez
Sep 23, 2008·Reproduction in Domestic Animals = Zuchthygiene·J M VazquezE A Martínez
Mar 1, 2012·Reproduction in Domestic Animals = Zuchthygiene·I ParrillaJ M Vazquez
Jun 28, 2015·Theriogenology·M SpinaciD Bucci
Sep 30, 2014·Theriogenology·Sylwia ProchowskaAgnieszka Partyka
Feb 7, 2018·Reproduction in Domestic Animals = Zuchthygiene·A PinyopumminP Wattananit
Jun 15, 2012·Reproduction in Domestic Animals = Zuchthygiene·I CaballeroJ M Vázquez
Jun 22, 2019·European Biophysics Journal : EBJ·Robert-William WelkePeter Müller
Jan 11, 2020·Frontiers in Veterinary Science·Cristina A MartinezManuel Álvarez-Rodriguez
Sep 15, 2020·Frontiers in Veterinary Science·Yanshe XieLinjun Hong
Sep 10, 2020·International Journal of Molecular Sciences·Lisa HöfnerDagmar Waberski
Apr 4, 2021·Animals : an Open Access Journal From MDPI·Estíbaliz LacalleFelipe Martínez-Pastor

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