Comparative effects of inorganic and organic nitrogen on the growth and microcystin production of Microcystis aeruginosa

World Journal of Microbiology & Biotechnology
YangWei YanXuanHao Wu

Abstract

Nitrogen causes the frequent occurrence of harmful algal blooms and possible microcystin production. The effects of ammonia and alanine (Ala) on the growth and microcystin production of Microcystis aeruginosa were investigated using an isotope tracer ((15)N). The results indicated that Ala was directly used by M. aeruginosa and contributed to biomass formation amounting to 2.1 × 10(7) cells mL(-1) on day 48, compared with only 6.2 × 10(6) cells mL(-1) from ammonia alone. Microcystin-LR production with Ala was less than that of ammonia, which peaked at 50.2 fg cell(-1) on day 6. Liquid chromatographic analysis with tandem mass spectrometry of (15)N-microcystin-LR suggested that (15)N from ammonia was probably synthesized into the arginine residue. By contrast, (15)N from Ala was assimilated into the Ala, leucine, the iso-linked (2R,3S)-3-methylaspartic acid, arginine, and certain unusual C20 amino acid residues. The results represent the forward steps in the determination of the nitrogen forms that fuel toxin production and blooms.

References

Jun 1, 1992·The Journal of Applied Bacteriology·W W Carmichael
Feb 3, 2005·Rapid Communications in Mass Spectrometry : RCM·Cariton KubwaboFrank M Benoit
Sep 7, 2005·Photosynthesis Research·M Isabel Muro-PastorFrancisco J Florencio
Jan 29, 2008·Toxicon : Official Journal of the International Society on Toxinology·Ruihua DaiYining Hou
Jan 12, 2011·Analytical and Bioanalytical Chemistry·Tomoharu SanoKunimitsu Kaya
Jun 16, 2011·Environmental Science & Technology·Amanda E PosteStephanie J Guildford
Oct 8, 2011·Science·Justin D Brookes, Cayelan C Carey
Oct 14, 2011·Microbial Ecology·Simoné DowningTimothy G Downing

❮ Previous
Next ❯

Citations

Feb 15, 2016·World Journal of Microbiology & Biotechnology·Ruihua DaiYifei Wang
May 30, 2019·International Journal of Molecular Sciences·Krystian Miazek, Beata Brozek-Pluska

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
Deon van der MerweEdward Carney
Science
Hans W PaerlSteven W Wilhelm
Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering
Hai YanMingming Zhang
© 2022 Meta ULC. All rights reserved