PMID: 6403378Apr 1, 1983Paper

Comparative effects of insulin and hydrocortisone on glycogen content of fetal and newborn rat liver cultures

Experimental and Molecular Pathology
G B Reed

Abstract

Organ cultures prepared from 18- to 19-day fetal and 3- to 6-day-old newborn rat liver were maintained for 2 days in Trowell's T8 medium without insulin and supplemented with 0.1% albumin and 300 mg% glucose. The atmosphere for culture was 95% O2 and 5% CO2. Medium alone was used for control cultures, whereas insulin, hydrocortisone, or insulin plus hydrocortisone were used in experimental groups. Explant glycogen stores were maintained better in cultures grown in hormone-supplemented media than in control cultures. Fetal explants were found to have higher levels of glycogen than controls in the insulin or insulin plus hydrocortisone groups. Postnatal explants did not have higher levels of glycogen in the hormone-treated groups. Gestational age appeared to determine whether the liver explants reacted to hydrocortisone or insulin to maintain glycogen stores. Enzymatic assays, in vitro of glycogen synthetase and phosphorylase, indicated that the fetal liver response to insulin plus hydrocortisone by increasing the total and independent form of glycogen synthetase; but similar enzyme studies on postnatal rat liver did not show convincing differences as to an effect on synthetase. No definite in vitro temporal relationships could b...Continue Reading

References

Mar 18, 1976·The New England Journal of Medicine·T D Gelehrter
Mar 4, 1976·The New England Journal of Medicine·T D Gelehrter
Mar 11, 1976·The New England Journal of Medicine·T D Gelehrter
Oct 1, 1978·Developmental Biology·R A SimkinsW H Glinsmann
Aug 1, 1975·The Biochemical Journal·P C MacDonnelO Greengard
Aug 1, 1973·The Biochemical Journal·A L Schwartz, T W Rall
Dec 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·H J EisenW H Glinsmann
May 1, 1974·The Biochemical Journal·P Devos, H G Hers
Jan 1, 1973·Pediatric Research·N C Räihä
Jun 1, 1974·The Journal of Clinical Investigation·B T SmithC J Giroud
Sep 1, 1971·Experimental Cell Research·A K Campbell, C N Hales
Dec 1, 1970·Clinica Chimica Acta; International Journal of Clinical Chemistry·F Huijing
Sep 1, 1969·Analytical Biochemistry·J B Lloyd, W J Whelan

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Citations

Jan 1, 1992·Virchows Archiv. A, Pathological Anatomy and Histopathology·R SchoberW Wechsler

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