PMID: 8967448May 1, 1996Paper

Comparative effects of insulin and isoproterenol on lipoprotein lipase in rat adipose cells

The American Journal of Physiology
G E Chiappe de CingalaniC I Caldiz

Abstract

The effects of insulin and isoproterenol on lipoprotein lipase mass and enzyme activity were investigated in rat adipocytes. Cells were pulse labeled for 1 h with [35S]methionine to measure immunoprecipitable lipoprotein lipase. The results showed that 80% of the newly synthesized enzyme was membrane associated and 20% was secreted into the cell incubation medium. Enzyme activity was mainly associated with lipoprotein lipase secreted into the medium. A 10-min incubation with 10(-7) M insulin stimulated the secretion of lipoprotein lipase activity and the activity associated with adipocyte membranes. Conversely, 10(-6) M isoproterenol decreased the activity in all fractions. In addition, insulin increased lipoprotein lipase mass associated with cell membranes and decreased that in the incubation medium, whereas isoproterenol induced a decrease in both cell membranes and medium. Insulin and isoproterenol stimulated phosphorylation of lipoprotein lipase. These findings suggest that insulin stimulates the secretion of active lipoprotein lipase and a reuptake of inactive secreted enzyme, and isoproterenol decreases the activity by enzyme degradation. Moreover, because both agents stimulate phosphorylation of lipoprotein lipase, phos...Continue Reading

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Citations

Mar 9, 2000·Diabetes/metabolism Research and Reviews·J J Gagliardino
Aug 16, 1997·Biochimica Et Biophysica Acta·M C BarberR G Vernon
Mar 12, 2004·Metabolism: Clinical and Experimental·Gladys E Chiappe De Cingolani, Claudia I Caldiz

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