Comparative effects of microplasmin and tissue-type plasminogen activator (tPA) on cerebral hemorrhage in a middle cerebral artery occlusion model in mice

Journal of Thrombosis and Haemostasis : JTH
Y SuzukiD Collen

Abstract

Thrombolytic therapy of ischemic stroke with tissue-type plasminogen activator (tPA) improves clinical outcome which may, however, be partially offset by significant intracerebral bleeding (ICB). The comparative effects of microplasmin (microPli) and tPA on ICB were evaluated in a thrombotic middle cerebral artery (MCA) occlusion model in mice. A dose of microPli (5 mg kg(-1)) or tPA (4 mg kg(-1)) which are comparably effective for reduction of brain damage, or a double dose (10 or 8 mg kg(-1), respectively) or the microPli excipient as a control were intravenously administered as a bolus at 30 min or 4 h after MCA occlusion. ICB was measured at 24 h by hemoglobin assay of exsanguinated brain extracts. Bleeding time was measured by tail cutting. In controls given solvent at 4 h, ICB was on average 8.8 micro L, which was significantly increased with 10 mg kg(-1) microPli and with 4 and 8 mg kg(-1) tPA to 12-13 micro L (P < 0.05 each vs. controls, n = 7-9), whereas 5 mg kg(-1) microPli did not affect bleeding (8.5 micro L P = NS vs. controls, n = 7). When given at 30 min, neither microPli nor tPA altered ICB (6.3-6.8 micro L, mean; n = 7-9). tPA but not microPli increased bleeding time; from 2.4 min in controls to 5.9 min (median...Continue Reading

References

Mar 15, 1978·European Journal of Biochemistry·B Wiman, D Collen
Dec 14, 1995·The New England Journal of Medicine·UNKNOWN National Institute of Neurological Disorders and Stroke rt-PA Stroke Study Group
Nov 22, 1997·Stroke; a Journal of Cerebral Circulation·T F ChoudhriD J Pinsky
Sep 7, 2002·Stroke; a Journal of Cerebral Circulation·Paul A LapchakJustin A Zivin
Jul 23, 2003·Journal of Thrombosis and Haemostasis : JTH·N NagaiD Collen
Aug 30, 1957·Annals of the New York Academy of Sciences·J L AMBRUSG L COLLINS
Mar 16, 2004·Stroke; a Journal of Cerebral Circulation·UNKNOWN IMS Study Investigators

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Citations

Feb 20, 2009·Journal of Thrombosis and Thrombolysis·Peter VerhammeMarguerite Stas
Sep 29, 2011·Nature Communications·Kazuhiko NakanoTakashi Ooshima
Aug 30, 2008·Blood·Marc TjwaPeter Carmeliet
Oct 2, 2012·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Yasuhiro Suzuki
Apr 22, 2010·Vascular Health and Risk Management·Jane Hoover-Plow
Jun 26, 2007·Neurological Research·Rune Skovgaard RasmussenGudrun Boysen
Jun 2, 2009·Journal of Vascular and Interventional Radiology : JVIR·Imo E HoeferJoris I Rotmans
Jul 30, 2015·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Yasuhiro SuzukiKazuo Umemura
Jun 29, 2007·Journal of Thrombosis and Haemostasis : JTH·Y SuzukiH R Lijnen
Dec 1, 2009·Journal of Thrombosis and Haemostasis : JTH·V J Marder, V Novokhatny
Jul 21, 2005·Neuroscience Letters·Dorothe BurggrafGerhard F Hamann
Jul 3, 2010·Journal of Pharmacological Sciences·Yasuhiro Suzuki
Oct 30, 2016·Bioengineered· Adivitiya, Yogender Pal Khasa
Nov 7, 2013·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Hironobu SawadaKeiji Hasumi
Jun 5, 2014·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Be'eri Niego, Robert L Medcalf
Jan 27, 2018·Arteriosclerosis, Thrombosis, and Vascular Biology·Marc PraetnerChristoph A Reichel
Jan 20, 2011·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·Agustín N Joison, Federico S Gallo
Jan 9, 2007·Biotechnology Letters·Zhifeng MaJian-Ning Liu
May 24, 2006·Expert Review of Cardiovascular Therapy·Nicole R Gonzales, James C Grotta
Oct 1, 2016·The British Journal of Ophthalmology·Marc D de SmetBart Jonckx
Jan 12, 2021·Frontiers in Cardiovascular Medicine·Satish SinghGuy L Reed
Jul 24, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Dong WangPeng Xu

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