Comparative effects of nodularin and microcystin-LR in zebrafish: 1. Uptake by organic anion transporting polypeptide Oatp1d1 (Slco1d1)

Aquatic Toxicology
Susanne FaltermannKarl Fent

Abstract

Microcystin-LR (MC-LR) and nodularin are hepatotoxins produced by several cyanobacterial species. Their toxicity is based on active cellular uptake and subsequent inhibition of protein phosphatases PP1/2A, leading to hyperphosphorylation and cell death. To date, uptake of MC-LR and nodularin in fish is poorly understood. Here, we investigated the role of the organic anion transporting polypeptide Oatp1d1 in zebrafish (drOatp1d1, Slco1d1) in cellular uptake in zebrafish. We stably transfected CHO and HEK293 cell lines expressing drOatp1d1. In both transfectants, uptake of MC-LR and nodularin was demonstrated by competitive inhibition of uptake with fluorescent substrate lucifer yellow. Direct uptake of MC-LR was demonstrated by immunostaining, and indirectly by the high cytotoxicity in stable transfectants. By means of a synthesized fluorescent labeled MC-LR derivative, direct uptake was further confirmed in HEK293 cells expressing drOatp1d1. Additionally, uptake and toxicity was investigated in the permanent zebrafish liver cell line ZFL. These cells had only a low relative abundance of drOatp1d1, drOatp2b1 and drOatp1f transcripts, which correlated with the lack of MC-LR induced cytotoxicity and transcriptional changes of gene...Continue Reading

References

Jun 29, 1999·Journal of Bacteriology·B A NeilanT Börner
Mar 4, 2000·Environmental Health Perspectives·B C HitzfeldD R Dietrich
May 20, 2000·Chemosphere·M W ChongW F Fong
Dec 31, 2002·Toxicology·Sandra M F O AzevedoGeoff K Eaglesham
Jul 23, 2003·Bioinformatics·Perikles Simon
Mar 2, 2005·Toxicology and Applied Pharmacology·C Wiegand, S Pflugmacher
Mar 2, 2005·Toxicology and Applied Pharmacology·W J FischerB Hagenbuch
Mar 21, 2007·Toxicological Sciences : an Official Journal of the Society of Toxicology·Masaharu KomatsuToru Takeuchi
May 15, 2007·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Constanze HilgendorfJohan Karlsson
Feb 26, 2008·Toxicological Sciences : an Official Journal of the Society of Toxicology·Hong LuCurtis D Klaassen
Nov 26, 2008·Toxicology and Applied Pharmacology·D FeursteinD R Dietrich
Nov 26, 2009·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Marta PopovicTvrtko Smital
Feb 1, 2011·Mutation Research·Bojana ZeguraMetka Filipič
Oct 22, 2011·British Journal of Pharmacology·Megan RothBruno Hagenbuch
Jul 23, 2013·Clinica Chimica Acta; International Journal of Clinical Chemistry·Yun ChenDanjun Fang
Oct 16, 2013·The Journal of Biological Chemistry·Marta PopovicTvrtko Smital
Sep 15, 2014·Toxicology and Applied Pharmacology·Konstanze SteinerDaniel R Dietrich
May 27, 2015·Chembiochem : a European Journal of Chemical Biology·Verena GrundlerKarl Gademann

❮ Previous
Next ❯

Citations

Nov 10, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Peter KollarJiří Pazourek
Jul 29, 2018·Environmental Toxicology and Chemistry·Raffael Alois Willi, Karl Fent
Dec 12, 2020·Marine Pollution Bulletin·Guoliang ChenTingzhang Hu
Feb 25, 2021·Journal of Applied Toxicology : JAT·Anna RymuszkaŁukasz Adaszek
Jun 1, 2020·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Virág SzékelyCsilla Özvegy-Laczka
May 22, 2018·Environmental Science & Technology·Kun Zhang, Yanbin Zhao
May 29, 2020·Journal of Natural Products·Julia MoschnyTimo H J Niedermeyer
Mar 15, 2020·Ecotoxicology and Environmental Safety·Guoliang ChenTingzhang Hu
Jan 20, 2020·The Science of the Total Environment·Huimin WeiZhi Wang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.

B-Cell Lymphoma

B-cell lymphomas include lymphomas that affect B cells. This subtype of cancer accounts for over 80% of non-Hodgkin lymphomas in the US. Here is the latest research.