Comparative enzymatic properties of GapB-encoded erythrose-4-phosphate dehydrogenase of Escherichia coli and phosphorylating glyceraldehyde-3-phosphate dehydrogenase.

The Journal of Biological Chemistry
S Boschi-MullerG Branlant

Abstract

GapB-encoded protein of Escherichia coli and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) share more than 40% amino acid identity. Most of the amino acids involved in the binding of cofactor and substrates to GAPDH are conserved in GapB-encoded protein. This enzyme shows an efficient non-phosphorylating erythrose-4-phosphate dehydrogenase activity (Zhao, G., Pease, A. J., Bharani, N., and Winkler, M. E. (1995) J. Bacteriol. 177, 2804-2812) but a low phosphorylating glyceraldehyde-3-phosphate dehydrogenase activity, whereas GAPDH shows a high efficient phosphorylating glyceraldehyde-3-phosphate dehydrogenase activity and a low phosphorylating erythrose-4-phosphate dehydrogenase activity. To identify the structural factors responsible for these differences, comparative kinetic and binding studies have been carried out on both GapB-encoded protein of Escherichia coli and GAPDH of Bacillus stearothermophilus. The KD constant of GapB-encoded protein for NAD is 800-fold higher than that of GAPDH. The chemical mechanism of erythrose 4-phosphate oxidation by GapB-encoded protein is shown to proceed through a two-step mechanism involving covalent intermediates with Cys-149, with rates associated to the acylation and deacylation proc...Continue Reading

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Citations

May 29, 2009·Journal of the Royal Society, Interface·Christoph Hold, Sven Panke
Apr 7, 2005·Applied and Environmental Microbiology·Isabelle Meynial-SallesPhilippe Soucaille
Dec 3, 2014·Proceedings of the National Academy of Sciences of the United States of America·Thibault BarbierJean-Jacques Letesson
Aug 24, 2005·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Tomohiro ShimadaAkira Ishihama
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Aug 24, 2013·Environmental Microbiology·Pablo I NikelVíctor de Lorenzo
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Mar 21, 2018·Proceedings of the National Academy of Sciences of the United States of America·Paul P LinJames C Liao
May 28, 2019·Frontiers in Molecular Biosciences·Björn RichtsFabian M Commichau
Apr 1, 2021·EcoSal Plus·Angela TramontiRoberto Contestabile
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