PMID: 8587761Nov 1, 1995Paper

Comparative evaluation of 99Tcm-Hynic-HSA and 99Tcm-MAG3-HSA as possible blood pool agents

Nuclear Medicine Communications
K VerbekeAlfons Verbruggen

Abstract

Two strategies have been used to increase the 99Tcm binding strength of human serum albumin (HSA) and thus enhance its blood retention. In a first approach, HSA was derivatized with a varying number of hydrazino nicotinyl (Hynic) side-chains using N-hydroxysuccinimidyl hydrazino nicotinate. Labelling of this albumin derivative with 99Tcm resulted in labelling yields of 90-95%. On the other hand, a 99Tcm-MAG3-HSA conjugate was prepared using the preformed chelate approach. In this way, non-specific binding of 99Tcm to HSA could be excluded. The in vitro stability of both 99Tcm-HSA derivatives was evaluated by cysteine challenge experiments and revealed a much higher stability for 99Tcm-Hynic-HSA than for 99Tcm-MAG3-HSA. The biological behaviour of the preparations was evaluated in mice and a rabbit using 125I-HSA as an internal biological standard. The blood retention of 99Tcm-MAG3-HSA decreased more rapidly than that of 125I-HSA in both animal species, whereas 99Tcm-Hynic-HSA seemed to provide a quasi-perfect 99Tcm-labelled analogue for 125I-HSA and 99Tcm-red blood cells (99Tcm-RBCs). In addition, the blood retention of 99Tcm-Hynic-HSA appeared to be similar to that of 99Tcm-RBCs in a volunteer. These results clearly indicate t...Continue Reading

Citations

Jan 12, 2002·Nuclear Medicine and Biology·Guozheng LiuDonald J Hnatowich
Sep 23, 2003·Nuclear Medicine and Biology·Kristin VerbekeAlfons Verbruggen
Nov 6, 1998·Nuclear Medicine and Biology·O K HjelstuenA M Verbruggen
Jul 1, 1997·Nuclear Medicine and Biology·P WinnardD J Hnatowich
May 22, 2008·Dalton Transactions : an International Journal of Inorganic Chemistry·M Bashir-Uddin SurfrazPhilip J Blower
Feb 24, 2007·Journal of Medicinal Chemistry·M Bashir-Uddin SurfrazPhilip J Blower
Feb 7, 2006·Nuclear Medicine and Biology·Jean-Luc VanderheydenDonald J Hnatowich
Nov 17, 2001·Seminars in Nuclear Medicine·S BanerjeeN Ramamoorthy
Jan 12, 2011·Nuclear Medicine and Biology·Rodrigo TeodoroRodrigo Guimarães Queiróz

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