Comparative Evaluation of Broad-Panel PCR Assays for the Detection of Gastrointestinal Pathogens in Pediatric Oncology Patients

The Journal of Molecular Diagnostics : JMD
Zhengming GuR T Hayden

Abstract

Broadly multiplexed molecular amplification assays offer an unprecedented ability to diagnose gastrointestinal infection in immunocompromised patients. However, little data are available to compare the performance of such systems in this population. A total of 436 stool samples were collected from 199 predominantly immunocompromised pediatric oncology patients. Remnant samples were tested in parallel with the use of the premarket (investigational use only) versions of two broadly multiplexed PCR assays (BioFire and Luminex), and the results of samples corresponding to the first episode per patient were compared with those from laboratory-developed molecular assays, culture, and antigen detection. Overall performance of the multiplexed systems was comparable, with BioFire and Luminex detecting 94 and 99 positives (P = 0.34), respectively. Stratifying by analyte, BioFire assay detected 51 samples positive for Clostridium difficile, whereas Luminex assay detected 60 (P = 0.01). Biofire and Luminex detected 28 and 38 norovirus-positive samples (P = 0.002), respectively. Astrovirus- and adenovirus-positive samples were detected in higher numbers by in-house PCR than by BioFire; the same was observed for adenovirus with Luminex. Diff...Continue Reading

References

Oct 9, 2003·Journal of Clinical Microbiology·Z GuR T Hayden
Jan 24, 2006·Journal of Virological Methods·Martin C W ChanWai K Leung
Jun 29, 2007·Journal of Clinical Microbiology·Frederick S NolteJames R Prudent
Apr 6, 2011·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Angela M Caliendo
Apr 22, 2011·Journal of Clinical Microbiology·Kenneth H RandHerbert J Houck
Feb 3, 2012·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Randall T HaydenMatthew J Bankowski
Oct 18, 2013·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·E WesselsE C J Claas
May 13, 2014·Seminars in Diagnostic Pathology·Keith K Lai, Laura W Lamps

❮ Previous
Next ❯

Citations

Feb 26, 2016·Diagnostic Microbiology and Infectious Disease·Jonas PettersonRosemary She
Nov 9, 2016·Gut Pathogens·Erica L TwitchellLijuan Yuan
Jan 14, 2017·Viruses·Philippe PérotMarc Eloit
Sep 1, 2016·Journal of the Pediatric Infectious Diseases Society·Mohammad N MhaissenElisabeth E Adderson
Jan 19, 2017·Emerging Infectious Diseases·Valerie CortezStacey Schultz-Cherry
Apr 22, 2017·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·C C OttoN E Babady

❮ Previous
Next ❯

Related Concepts

Related Feeds

Amoebiasis

Amoebiasis, infection by the protozoan parasite Entamoeba histolytica, remains a global health problem, despite the availability of effective treatment. Here is the latest research.