Abstract
Broadly multiplexed molecular amplification assays offer an unprecedented ability to diagnose gastrointestinal infection in immunocompromised patients. However, little data are available to compare the performance of such systems in this population. A total of 436 stool samples were collected from 199 predominantly immunocompromised pediatric oncology patients. Remnant samples were tested in parallel with the use of the premarket (investigational use only) versions of two broadly multiplexed PCR assays (BioFire and Luminex), and the results of samples corresponding to the first episode per patient were compared with those from laboratory-developed molecular assays, culture, and antigen detection. Overall performance of the multiplexed systems was comparable, with BioFire and Luminex detecting 94 and 99 positives (P = 0.34), respectively. Stratifying by analyte, BioFire assay detected 51 samples positive for Clostridium difficile, whereas Luminex assay detected 60 (P = 0.01). Biofire and Luminex detected 28 and 38 norovirus-positive samples (P = 0.002), respectively. Astrovirus- and adenovirus-positive samples were detected in higher numbers by in-house PCR than by BioFire; the same was observed for adenovirus with Luminex. Diff...Continue Reading
References
Oct 9, 2003·Journal of Clinical Microbiology·Z GuR T Hayden
Jan 24, 2006·Journal of Virological Methods·Martin C W ChanWai K Leung
Jun 29, 2007·Journal of Clinical Microbiology·J MahonyR Janeczko
Jun 29, 2007·Journal of Clinical Microbiology·Frederick S NolteJames R Prudent
Jan 7, 2009·Gastroenterology·James E Everhart, Constance E Ruhl
Apr 18, 2009·Leukemia & Lymphoma·Sujatha MuraliAngela M Caliendo
Apr 2, 2010·Journal of Clinical Microbiology·Naomi J GadsbyKate E Templeton
Apr 6, 2011·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Angela M Caliendo
Apr 22, 2011·Journal of Clinical Microbiology·Kenneth H RandHerbert J Houck
Nov 1, 2011·PloS One·Mark A PoritzKirk M Ririe
Feb 3, 2012·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Randall T HaydenMatthew J Bankowski
May 29, 2013·Journal of Microbiology and Biotechnology·Eric C ClaasFrancois Topin
Jul 13, 2013·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Ellen Jo BaronBobbi S Pritt
Jul 16, 2013·Journal of Clinical Microbiology·Jose F NavidadSanjib Bhattacharyya
Jul 25, 2013·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Ellen Jo BaronBobbi S Pritt
Oct 15, 2013·Pediatric Blood & Cancer·Ashok SrinivasanRandall T Hayden
Oct 18, 2013·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·E WesselsE C J Claas
May 13, 2014·Seminars in Diagnostic Pathology·Keith K Lai, Laura W Lamps
Aug 8, 2014·Journal of Clinical Microbiology·Reeti KhareMatthew J Binnicker
Dec 3, 2014·The Journal of Infection·Simon D GoldenbergJonathan D Edgeworth
Citations
Feb 26, 2016·Diagnostic Microbiology and Infectious Disease·Jonas PettersonRosemary She
Nov 9, 2016·Gut Pathogens·Erica L TwitchellLijuan Yuan
Jan 14, 2017·Viruses·Philippe PérotMarc Eloit
Sep 1, 2016·Journal of the Pediatric Infectious Diseases Society·Mohammad N MhaissenElisabeth E Adderson
Mar 3, 2017·PloS One·Karoline FreemanJames Mason
Jul 22, 2016·Journal of Clinical Microbiology·Bobbi S PrittRichard B Thomson
Nov 4, 2017·PloS One·Izaskun Alejo-CanchoMª Ángeles Marcos
Apr 12, 2019·Journal of Virology·Valerie CortezStacey Schultz-Cherry
Jul 9, 2020·Pediatric Transplantation·John M StoneScott Curry
Jun 18, 2017·Health Technology Assessment : HTA·Karoline FreemanJames Mason
Jan 19, 2017·Emerging Infectious Diseases·Valerie CortezStacey Schultz-Cherry
Apr 22, 2017·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·C C OttoN E Babady
Feb 11, 2020·Clinical Chemistry·Gillian A M TarrStephen B Freedman
Aug 4, 2021·Analytical and Bioanalytical Chemistry·Changyoon BaekJunhong Min