Comparative evaluation of chemical and enzymatic saccharification of mixotrophically grown de-oiled microalgal biomass for reducing sugar production

Bioresource Technology
Imran PanchaSandhya Mishra

Abstract

For the commercialization of microalgal based biofuels, utilization of de-oiled carbohydrate rich biomass is important. In the present study, chemo-enzymatic hydrolysis of mixotrophically grown Scenedesmus sp. CCNM 1077 de-oiled biomass is evaluated. Among the chemical hydrolysis, use of 0.5M HCl for 45 min at 121°C resulted in highest saccharification yield of 37.87% w/w of de-oiled biomass. However, enzymatic hydrolysis using Viscozyme L at loading rate of 20 FBGU/g of de-oiled biomass, pH 5.5 and temperature 45°C for 72 h resulted in saccharification yield of 43.44% w/w of de-oiled biomass. Further, 78% ethanol production efficiency was achieved with enzymatically hydrolyzed de-oiled biomass using yeast Saccharomyces cerevisiae ATCC 6793. These findings of the present study show application of mixotrophically grown de-oiled biomass of Scenedesmus sp. CCNM 1077 as promising feedstock for bioethanol production.

References

Mar 14, 2007·Biotechnology Advances·Yusuf Chisti
Mar 12, 2010·Bioresource Technology·Seung Phill ChoiSang Jun Sim
Apr 27, 2010·International Journal of Biological Macromolecules·Imran PanchaBhavanath Jha
Jun 30, 2010·Bioresource Technology·Ramesh Chander KuhadAjay Singh
Aug 14, 2010·Science·René H Wijffels, Maria J Barbosa
Nov 19, 2011·Bioresource Technology·J R MirandaL Gouveia
Nov 15, 2013·Biotechnology Journal·Razif HarunMichael K Danquah

❮ Previous
Next ❯

Citations

May 11, 2016·Bioresource Technology·Rahulkumar MauryaSandhya Mishra
Sep 20, 2016·Bioresource Technology·Rahulkumar MauryaSandhya Mishra
Jul 19, 2019·Water Science and Technology : a Journal of the International Association on Water Pollution Research·William MichelonMárcio Luís Busi da Silva
Jan 21, 2021·International Journal of Biological Macromolecules·Jorge Lucas da MaiaMichele Greque de Morais

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biofuels (ASM)

Biofuels are produced through contemporary processes from biomass rather than geological processes involved in fossil fuel formation. Examples include biodiesel, green diesel, biogas, etc. Discover the latest research on biofuels in this feed.