Comparative evaluation of four transport media for maintaining cell viability in transportation of an avulsed tooth - An in vitro study

Journal of International Society of Preventive & Community Dentistry
Makonahalli Jaganath BharathAshna Thomas

Abstract

The study was performed to compare and evaluate the efficacy of four experimental storage media (Hank's balanced salt solution, Ringer's lactate solution, tender coconut water, and green tea extract) for maintaining cell viability of human periodontal cells at different time intervals of 15 min 30 min, 60 min, and 90 min. Human periodontal cells were cultured and stored in the four media. After 15 min 30 min, 60 min, and 90 min, the different media were examined under optical microscope and viabilities analyzed using an optical calorimeter. Mean and standard deviation were estimated from the results that were statistically analyzed using one-way analysis of variance (ANOVA) to identify the significant groups. The results indicated that there was no difference in cell viability between the four media up to a period of 60 min, whereas green tea extract showed a lower cell viability after 90 min. Within the limitations of the present study, it appears that due to superior osmolality, cost effectiveness, and easier availability, Ringer's lactate, tender coconut water, and green tea extract can be used as alternate storage media for avulsed tooth.

References

Mar 1, 1977·Scandinavian Journal of Dental Research·P O SöderT Modéer
Dec 1, 1983·Scandinavian Journal of Dental Research·S LindskogL Hammarström
Apr 1, 1995·Endodontics & Dental Traumatology·J O AndreasenF M Andreasen
Feb 12, 2009·Journal of Korean Medical Science·Byung Chul ShinHyun Lee Kim
Jun 22, 2011·Journal of Endodontics·Ji Young HwangSang Wook Kang
Mar 26, 2013·Journal of Endodontics·Maíra PradoBrenda P F A Gomes

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Citations

Jan 3, 2018·Dental Traumatology : Official Publication of International Association for Dental Traumatology·Samira AdnanSana E Nagi
Jul 23, 2019·Dental Traumatology : Official Publication of International Association for Dental Traumatology·Kêmelly Karolliny Moreira ResendeCláudio Rodrigues Rezende Costa

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Methods Mentioned

BETA
scraping

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