Comparative evaluation of half-maximum inhibitory concentration and cytotoxicity of silver nanoparticles and multiwalled carbon nanotubes using buffalo bull spermatozoa as a cell model

Toxicology and Industrial Health
Sandhya SanandGautam Kaul

Abstract

There is a dearth of information regarding the safety of silver nanoparticles (Ag NPs) and multiwalled carbon nanotubes (MWCNTs) with respect to their impact on human/animal health and the environment. This study aimed to determine the half-maximum inhibitory concentration (IC50) of Ag NPs and MWCNTs by employing different doses and time interval combinations in buffalo bull spermatozoa. Semen samples containing 100 million spermatozoa each were incubated with 1, 10, 25, 50, 75 and 100 µg/mL of Ag NPs and MWCNTs at 37°C for 30, 60 and 120 min. Sperm viability was monitored by the MTT assay and eosin-nigrosin staining followed by estimation of IC50 values using correlation-regression analysis. Spermatozoa treated with IC50 doses of Ag NPs and MWCNTs were also assessed for different sperm functionality parameters including oxidative stress and membrane integrity. These parameters were observed to be significantly affected in treated spermatozoa compared with the controls. We concluded that both nanomaterials showed cytotoxicity, mediated principally via oxidative stress. This work has provided valuable toxicological information that will serve as a benchmark for future studies aimed at safe use of nanomaterials.

References

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Citations

Mar 20, 2021·The Science of the Total Environment·Maingredy Rodrigues SouzaThiago Lopes Rocha
May 1, 2021·Veterinary Research Communications·Syed Aftab Hussain Shah, Syed Murtaza Hassan Andrabi

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Methods Mentioned

BETA
dynamic light scattering
scanning electron microscopy
transmission electron microscopy
MDA
Phase

Software Mentioned

GraphPad prism

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