Comparative evaluation of human embryonic stem cell lines derived from zygotes with normal and abnormal pronuclei

Developmental Dynamics : an Official Publication of the American Association of Anatomists
Qing HuanZi-Jiang Chen

Abstract

Human embryonic stem (hES) cell lines have been derived from normally or abnormally fertilized zygotes. However, the similar and different properties of these two types of hES cell lines are not well-known. To address this question, we generated nine hES cell lines from zygotes containing normal (2PN) and abnormal (0PN, 1PN, 3PN) pronuclei. A side-by-side comparison showed that all cell lines exhibited distinct identity and karyotypical stability. They expressed similar "stemness" markers and alkaline phosphatase activity and differentiated into three embryonic germ lineages in embryoid bodies and teratomas. Under neural differentiation-promoting conditions, they were directed into neural progenitors and neurons. However, a variation in cell cycle and the relative abundance of gene expression of undifferentiated and differentiated markers were observed. These variations were also seen among individually derived normal hES cell lines. Thus, normal hES cell lines can be developed from fertilized zygotes with abnormal pronuclei usually excluded from clinical use.

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Citations

Apr 25, 2012·Journal of Assisted Reproduction and Genetics·Xinjie ChenXiaofang Sun
Feb 24, 2016·Systems Biology in Reproductive Medicine·Bao-Li YinCui-Lian Zhang
Apr 9, 2013·Journal of Cellular Biochemistry·Chunyan JiangJiayin Liu
Mar 8, 2016·Molecular Reproduction and Development·Yi-Fan GuGuang-Xiu Lu
Jan 5, 2019·Stem Cells and Development·Marjan OmidiMohammad Ali Khalili
Jun 17, 2021·Human Reproduction Update·Giovanni CoticchioAlison Campbell

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