Abstract
A simple screening method for fluconazole susceptibility using CHROMagar Candida with fluconazole was compared with the National Committee for Clinical Laboratory Standards (NCCLS) macrobroth method. In this agar dilution method, susceptible Candida albicans colonies are smaller on medium with fluconazole than on fluconazole-free medium. Yeasts with decreased susceptibility have normal-sized colonies on medium containing fluconazole. On agar with 16 micrograms of fluconazole per ml, 32 of 34 strains with NCCLS MICs of > or = 16 micrograms/ml were correctly predicted, as were 66 of 68 with MICs of < 16, an agreement of 96%. On agar with 8 micrograms of fluconazole per ml, 38 of 41 isolates with MICs of > or = 8 were correctly predicted, as were 59 of 61 isolates with MICs of < 8, an agreement of 95%. This agar dilution methods appears to highly correlate with NCCLS macrobroth methods for detection of C. albicans and may be an effective screen for fluconazole susceptibility.
References
May 1, 1995·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·B LouwagieF Odds
Jan 1, 1995·Antimicrobial Agents and Chemotherapy·J H RexM A Pfaller
Jan 1, 1995·Antimicrobial Agents and Chemotherapy·J H RexC D Webb
Oct 1, 1994·Journal of Clinical Microbiology·F BarchiesiM G Rinaldi
Jan 1, 1994·Journal of Clinical Microbiology·M A PfallerM G Rinaldi
Aug 1, 1994·Journal of Clinical Microbiology·F C Odds, R Bernaerts
Apr 1, 1994·Journal of Clinical Microbiology·L MillonY Michel-Briand
Feb 1, 1994·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·S ReddingM Pfaller
Oct 1, 1993·Clinical Microbiology Reviews·J H RexJ N Galgiani
Jan 1, 1993·Antimicrobial Agents and Chemotherapy·R A FromtlingG D Roberts
Jan 1, 1996·The Journal of Infectious Diseases·J R MaenzaJ E Gallant
Jan 1, 1996·Journal of Clinical Microbiology·M A PfallerS Coffmann
Jul 1, 1996·Journal of Clinical Microbiology·T F PattersonM G Rinaldi
Oct 1, 1996·The Journal of Infectious Diseases·S G RevankarT F Patterson
Citations
Apr 8, 2003·Journal of Chemotherapy·N EsenN Yuluğ
May 16, 2007·Antimicrobial Agents and Chemotherapy·Palani PerumalW LaJean Chaffin
Feb 5, 2013·Journal of Medical Microbiology·Sean G ChadwickScott E Gygax
Sep 15, 2001·Antimicrobial Agents and Chemotherapy·S PereaT F Patterson
Apr 8, 2010·Medical Mycology·Steven D WestbrookSpencer W Redding
Oct 5, 2001·Clinical Microbiology Reviews·J H RexD W Warnock
Jan 30, 2010·Medical Mycology·William R KirkpatrickSpencer W Redding
Jul 2, 1999·Antimicrobial Agents and Chemotherapy·J L Lopez-RibotT F Patterson
Oct 31, 1998·Antimicrobial Agents and Chemotherapy·J L Lopez-RibotT F Patterson
Sep 3, 1999·Clinical and Diagnostic Laboratory Immunology·J L Lopez-RibotT F Patterson
May 8, 2003·Journal of Clinical Microbiology·Susan M Nelson, Charles P Cartwright
Oct 17, 1998·Journal of Clinical Microbiology·W R KirkpatrickT F Patterson
May 9, 1998·Journal of Clinical Microbiology·W R KirkpatrickT F Patterson
Feb 12, 1998·Journal of Clinical Microbiology·S G RevankarT F Patterson
May 1, 2002·Journal of Clinical Microbiology·Spencer W ReddingThomas F Patterson
Aug 31, 2002·Journal of Clinical Microbiology·Marcos MartinezThomas F Patterson