Comparative evaluation of macrodilution and chromogenic agar screening for determining fluconazole susceptibility of Candida albicans.

Journal of Clinical Microbiology
T F PattersonM G Rinaldi

Abstract

A simple screening method for fluconazole susceptibility using CHROMagar Candida with fluconazole was compared with the National Committee for Clinical Laboratory Standards (NCCLS) macrobroth method. In this agar dilution method, susceptible Candida albicans colonies are smaller on medium with fluconazole than on fluconazole-free medium. Yeasts with decreased susceptibility have normal-sized colonies on medium containing fluconazole. On agar with 16 micrograms of fluconazole per ml, 32 of 34 strains with NCCLS MICs of > or = 16 micrograms/ml were correctly predicted, as were 66 of 68 with MICs of < 16, an agreement of 96%. On agar with 8 micrograms of fluconazole per ml, 38 of 41 isolates with MICs of > or = 8 were correctly predicted, as were 59 of 61 isolates with MICs of < 8, an agreement of 95%. This agar dilution methods appears to highly correlate with NCCLS macrobroth methods for detection of C. albicans and may be an effective screen for fluconazole susceptibility.

References

May 1, 1995·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·B LouwagieF Odds
Jan 1, 1995·Antimicrobial Agents and Chemotherapy·J H RexM A Pfaller
Feb 1, 1994·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·S ReddingM Pfaller
Oct 1, 1993·Clinical Microbiology Reviews·J H RexJ N Galgiani
Jan 1, 1993·Antimicrobial Agents and Chemotherapy·R A FromtlingG D Roberts
Jan 1, 1996·The Journal of Infectious Diseases·J R MaenzaJ E Gallant
Jul 1, 1996·Journal of Clinical Microbiology·T F PattersonM G Rinaldi

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Citations

May 16, 2007·Antimicrobial Agents and Chemotherapy·Palani PerumalW LaJean Chaffin
Feb 5, 2013·Journal of Medical Microbiology·Sean G ChadwickScott E Gygax
Oct 5, 2001·Clinical Microbiology Reviews·J H RexD W Warnock
Jan 30, 2010·Medical Mycology·William R KirkpatrickSpencer W Redding
May 8, 2003·Journal of Clinical Microbiology·Susan M Nelson, Charles P Cartwright
Oct 17, 1998·Journal of Clinical Microbiology·W R KirkpatrickT F Patterson
May 1, 2002·Journal of Clinical Microbiology·Spencer W ReddingThomas F Patterson

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