Comparative evaluation of post-column free radical scavenging and ferric reducing antioxidant power assays for screening of antioxidants in strawberries

Journal of Chromatography. a
Raimondas RaudonisValdimaras Janulis

Abstract

ABTS and FRAP post-column techniques evaluate the antioxidant characteristics of HPLC separated compounds with specific reagents. ABTS characterize their ability to scavenge free radicals by electron-donating antioxidants, resulting in the absorbance decrease of the chromophoric radical. FRAP - is based on the reduction of Fe(III)-tripyridyltriazine complex to Fe(II)-tripyridyltriazine at low pH by electron-donating antioxidants, resulting in an absorbance increase. Both post-column assays were evaluated and compared according to the following validation parameters: specificity, precision, limit of detection (LoD), limit of quantitation (LoQ) and linearity. ABTS and FRAP post-column assays were specific, repeatable and sensitive and thus can be used for the evaluation of antioxidant active compounds. Antioxidant active compounds were quantified according to TEAC for each assay and ABTS/FRAP ratio was derived. No previous records of antioxidative activity of leaves and fruits of strawberries (Fragaria viridis, Fragaria moschata) research have been found. The research results confirm the reliability of ABTS and FRAP post-column assays for screening of antioxidants in complex mixtures and the determination of radical scavenging an...Continue Reading

References

May 2, 2002·Journal of Agricultural and Food Chemistry·Audrius PukalskasAede de Groot
May 12, 2005·Journal of Agricultural and Food Chemistry·Kjersti AabyRonald E Wrolstad
Jun 7, 2007·Journal of Agricultural and Food Chemistry·Kjersti AabyGrete Skrede

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Citations

Apr 4, 2015·Natural Product Research·Mindaugas MarksaRūta Marksienė
Mar 24, 2016·Journal of Food Science·Lina RaudoneValdimaras Janulis
Aug 4, 2015·Journal of Analytical Methods in Chemistry·Laura RimkieneValdas Jakstas
Mar 19, 2014·Natural Product Research·Raimondas RaudonisValdimaras Janulis
Apr 29, 2014·Preventive Nutrition and Food Science·Sadaf YaqoobMuhammad Mushtaq
Jun 21, 2019·Antioxidants·Guo-Yi TangHua-Bin Li
Mar 11, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Lauryna PudziuvelyteJurga Bernatoniene
Oct 14, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Guo-Yi TangHua-Bin Li
Aug 28, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Lina RaudonePranas Viskelis

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