PMID: 9432320Mar 1, 1997Paper

Comparative evaluation of selective and nonselective media for primary isolation of Helicobacter pylori from gastric biopsies

Helicobacter
M J Fresnadillo MartínezJ A García-Rodríguez

Abstract

In this study, we compared and evaluated the efficacy of five culture media for the primary isolation of Helicobacter pylori from gastric biopsies. A total of 1,174 biopsies (antrum and corpus) taken from 587 patients were plated in parallel on two selective media--Skirrow's medium and brain-heart infusion (BHI) agar supplemented with 10% sheep blood, polymyxin B, vancomycin, trimethoprim, and amphotericin B (HPA medium), and on three nonselective media--chocolate agar with Isovitalex, Columbia blood agar, and BHI-10% sheep blood agar. An isolation rate of 57.9% (680 of 1,174) was obtained with a combination of all media. HPA medium gave the highest isolation rate, 99.4% (676 of 680). Chocolate agar, Columbia blood agar, brain-heart blood agar, and Skirrow's medium showed very poor performance (23.5%, 28.5%, 65.9%, and 71% of all isolates, respectively). The number of cultures in HPA medium discarded due to contamination was only 2 (0.32%). There was no difference in the positive rate of culture in HPA medium between the antrum and the corpus of the stomach. The HPA medium is superior to Skirrow's medium and nonselective media in promoting growth of H. pylori and, on the basis of these results, we recommended the use of HPA med...Continue Reading

References

May 1, 1992·Journal of Clinical Microbiology·L CelliniB Dainelli
Apr 1, 1991·Journal of Clinical Microbiology·P Nedenskov-SørensenG Bukholm
Apr 1, 1991·Journal of Clinical Microbiology·T U WestblomB R Midkiff
Jan 1, 1991·Journal of Clinical Microbiology·R AnsorgE N Schmid
Jan 1, 1990·Reviews of Infectious Diseases·J S Barthel, E D Everett
Jul 1, 1989·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·S L HazellD Y Graham
Aug 1, 1988·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·J C Dent, C A McNulty
Dec 1, 1987·Journal of Clinical Microbiology·D M QueirozG A Rocha
Apr 1, 1987·Journal of Clinical Microbiology·G E Buck, J S Smith
Nov 1, 1987·Journal of Clinical Microbiology·D R MorganW G Kraft
Aug 1, 1995·Journal of Clinical Pathology·C Y HachemD Y Graham
Feb 1, 1995·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·M L HänninenE Westermarck
Oct 1, 1993·Journal of Clinical Microbiology·D A FergusonE Thomas
Jan 1, 1993·Journal of Clinical Microbiology·R OlivieriJ Aziz
Nov 1, 1995·Academic Emergency Medicine : Official Journal of the Society for Academic Emergency Medicine·R F EdlichS D London
Nov 1, 1995·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·T H HenriksenT Madebo
Feb 1, 1997·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·L MonteiroF Mégraud

❮ Previous
Next ❯

Citations

Apr 30, 2003·Journal of Pediatric Gastroenterology and Nutrition·Roland N NdipLawrence T Weaver
Aug 30, 2005·Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research·Francisco J García-CriadoAlberto Gomez-Alonso
Nov 13, 2002·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·E CuchíS Quintana

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology
H RautelinT U Kosunen
European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology
D JonkersR Stockbrügger
Journal of Clinical Pathology
D CumminsS C Davies
© 2021 Meta ULC. All rights reserved