Comparative evaluation of the Aptima HIV-1 Quant Dx assay and COBAS TaqMan HIV-1 v2.0 assay using the Roche High Pure System for the quantification of HIV-1 RNA in plasma

Clinical Chemistry and Laboratory Medicine : CCLM
Gunnar SchalastaMartin Enders

Abstract

Quantification of human immunodeficiency virus type 1 (HIV-1) RNA in plasma has become the standard of care in the management of HIV-infected patients. There are several commercially available assays that have been implemented for the detection of HIV-1 RNA in plasma. Here, the new Hologic Aptima® HIV-1 Quant Dx assay (Aptima HIV) was compared to the Roche COBAS® TaqMan® HIV-1 Test v2.0 for use with the High Pure System (HPS/CTM). The performance characteristics of the assays were assessed using commercially available HIV reference panels, dilution of the WHO 3rd International HIV-1 RNA International Standard (WHO-IS) and plasma from clinical specimens. Assay performance was determined by linear regression, Deming correlation analysis and Bland-Altman analysis. Testing of HIV-1 reference panels revealed excellent agreement. The 61 clinical specimens quantified in both assays were linearly associated and strongly correlated. The Aptima HIV assay offers performance comparable to that of the HPS/CTM assay and, as it is run on a fully automated platform, a significantly improved workflow.

References

Jul 1, 1994·AIDS Research and Human Retroviruses·W JanssensC Atende
Jun 15, 1997·Annals of Internal Medicine·J W MellorsC R Rinaldo
Mar 21, 2002·Expert Review of Molecular Diagnostics·C S Hill
Oct 5, 2010·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Celine S YanPhilip H Cunningham
Nov 12, 2010·Journal of Clinical Microbiology·Thomas BourletPatrice André
Apr 26, 2012·BMC Infectious Diseases·Jean RuellePatrick Goubau
Apr 27, 2012·Journal of Clinical Microbiology·Michael T PyneDavid R Hillyard
Feb 2, 2013·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Ana Garcia-DiazAnna Maria Geretti

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Citations

Feb 21, 2016·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Sangeetha Vijaysri NairAndrew Worlock
Oct 12, 2016·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·K SaunéJ Izopet
Aug 19, 2016·Journal of Clinical Microbiology·Malaya K SahooBenjamin A Pinsky
Aug 12, 2016·Journal of Clinical Microbiology·Mark M ManakLinda L Jagodzinski
Jun 9, 2017·Journal of Clinical Microbiology·Christina YekDouglas D Richman

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Methods Mentioned

BETA
PCR
Assay

Software Mentioned

Instand
Aptima
GraphPad
GraphPad Prism
Panther
Aptima HIV
Aptima Quant Dx
Qnostics

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