Comparative evaluation of the new xTAG GPP multiplex assay in the laboratory diagnosis of acute gastroenteritis. Clinical assessment and potential application from a multicentre Italian study

International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases
C VocaleV Sambri

Abstract

Gastroenteritis caused by a single pathogen or multiple pathogens remains a major diagnostic challenge for the laboratory. The treatment of diarrhoea is based on microbiological results. Diagnosis is achieved using different laboratory techniques that have variable sensitivity and specificity. xTAG GPP is a new multiplex PCR assay that simultaneously detects 15 different pathogens responsible for diarrhoea. The results of the first multicentre study in Italy to evaluate the potential clinical application of the GPP assay in the laboratory diagnosis of diarrhoea are reported here. Faeces specimens (N=664) from hospitalized patients were tested with the GPP assay using a Luminex 200 instrument. All specimens were run using comparator methods following a routine algorithm: culture for bacteria, enzyme immunoassay and PCR for viruses, and microscopy for parasites. Of the samples tested with the GPP, 53.61% (356/664) gave positive results, as compared to 45.33% by routine testing. Of the positive specimens, 34.55% showed the presence of genomic DNA from multiple pathogens. The Luminex method showed an increase in the percentage of positivity of 8.28%. The GPP assay can be considered a helpful tool for the detection of gastrointestin...Continue Reading

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Nov 19, 2015·Expert Review of Molecular Diagnostics·Robert DuncanMing Dong
Feb 26, 2016·Diagnostic Microbiology and Infectious Disease·Jonas PettersonRosemary She
May 6, 2016·Frontiers in Microbiology·Brendon D ParsonsLinda Chui
Jun 24, 2016·The Open Microbiology Journal·Haifeng Chen, Yuan Hu
Dec 14, 2017·Journal of Medical Virology·Rainer GosertHans H Hirsch
Jun 16, 2021·Journal of Gastroenterology and Hepatology·Readon TehKewin Tien Ho Siah

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