Comparative evaluation of the Vitek 2 yeast susceptibility test and CLSI broth microdilution reference method for testing antifungal susceptibility of invasive fungal isolates in Italy: the GISIA3 study.

Journal of Clinical Microbiology
Elisa BorghiGiulia Morace

Abstract

The newly available AST-YS01 Vitek 2 cards were evaluated, and the results were compared with those obtained by the CLSI M27-A2 microdilution reference method. Clinical fungal isolates, including 614 isolates of Candida spp., 10 Cryptococcus neoformans isolates, 1 Geotrichum capitatum isolate, and 2 quality control strains, were tested for their susceptibilities to amphotericin B, fluconazole, and voriconazole using both methods. The majority of fungal isolates were susceptible to all antifungal agents tested: the MIC(90) values determined by the Vitek 2 and CLSI methods were 0.5 and 1 microg/ml, respectively, for amphotericin B; 8 and 16 microg/ml, respectively, for fluconazole; and <0.12 and 0.25 microg/ml, respectively, for voriconazole. Overall there was excellent categorical agreement (CA) between the methods (99.5% for amphotericin B, 92% for fluconazole, 98.2% for voriconazole), but discrepancies were observed within species. The CAs for fluconazole were low for Candida glabrata and Candida krusei when the results of the CLSI method at 48 h were considered. Moreover, the fully automated commercial system did not detect the susceptibility of Cryptococcus neoformans to voriconazole. The Vitek 2 system can be considered a v...Continue Reading

References

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Citations

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Sep 20, 2012·Medical Mycology·Jenny Wan Sai Cheong, Joe McCormack
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Jun 15, 2013·Revista iberoamericana de micología·Arnaldo Lopes ColomboUNKNOWN Grupo Proyecto Épico
Jun 15, 2013·Revista iberoamericana de micología·Arnaldo Lopes ColomboUNKNOWN Latin America Invasive Mycosis Network
Jan 8, 2016·Infectious Disease Clinics of North America·Mohammad T AlbatainehNathan P Wiederhold
Mar 18, 2016·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·S AroraR Kaur
Feb 3, 2015·Revista iberoamericana de micología·Tatiane BenaducciMaria José Soares Mendes-Giannini
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Feb 9, 2012·Memórias do Instituto Oswaldo Cruz·Marcos Dornelas-RibeiroSergio Eduardo Longo Fracalanzza
Jul 23, 2014·Antimicrobial Agents and Chemotherapy·George PankeyAbdulrahim Ismail
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