Comparative evaluation of three supplements for Helicobacter pylori growth in liquid culture.

Current Microbiology
Masoumeh DouraghiMarjan Mohammadi

Abstract

Helicobacter pylori, a microaerophilic fastidious bacterium, has been cultured on various plating and broth media since its discovery. Although the agar media can be sufficient for the identification, typing, and antibiotic resistance studies, no secretory antigen of H. pylori can be evaluated in such media. Thus, satisfactory growth of H. pylori in liquid culture which is needed for analysis of secretory proteins without the presence of interfering agents is in demand. We assessed the impact of beta-cyclodextrin, Fetal Bovine Serum (FBS), and charcoal as supplements for H. pylori growth. Furthermore, we aimed to identify the most favorable supplement that supports the secretion of the dominant secretory protein, vacuolating cytotoxin (VacA). Five clinical strains were cultured on broth media and the growth, viability, morphology, and protein content of each strain were determined. Our results revealed that beta-cyclodextrin supports the growth rate, viability, and cell lysate protein content to the extent similar to FBS. Application of beta-cyclodextrin is found to postpone spiral to coccoid conversion up to 72 h of incubation. Although FBS supports a higher VacA protein content, presence of interfering macromolecules in FBS q...Continue Reading

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Citations

Mar 13, 2012·Helicobacter·Luisa F Jiménez-SotoRainer Haas
Dec 15, 2012·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Lucinda J BessaLuigina Cellini
Nov 26, 2010·Helicobacter·Xavier CalvetFrancis Mégraud
May 6, 2014·Brazilian Journal of Anesthesiology·Volkan HanciAhmet Unver
Jan 10, 2013·International Journal of Immunopathology and Pharmacology·L J BessaI Rocha

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