Comparative expression profile of microRNAs and piRNAs in three ruminant species testes using next-generation sequencing

Reproduction in Domestic Animals = Zuchthygiene
H WangJ Xin

Abstract

microRNA (miRNA) and piwi-interacting RNA (piRNA) are two classes small non-coding regulatory RNAs that play crucial roles in multiple biological processes such as spermatogenesis. However, there are no published studies on conjoint analysis of miRNA and piRNA profiles among cattle, yak and their interspecies (the dzo) using sequencing technology. Next-generation sequencing technology was used to profile miRNAs and piRNAs among those three ruminants to elucidate their functions. A total of 119, 14 and six differentially expressed miRNAs were obtained in cattle vs. dzo, cattle vs. yak and yak vs. dzo comparison groups, while there were 873, 1,065 and 1,158 differentially expressed piRNAs in those three comparison groups. The expression of three miRNAs was validated in the three ruminants, and the results suggested that the miRNA expression profiles data could represent actual miRNA expression levels. Moreover, the putative targets of differentially expressed miRNAs were predicted by their own genome, it is worth to note that both the cattle and yak genome were used for dzo, then the targets were subjected to GO enrichment and KEGG pathway analysis, revealing the likely roles for these differentially expressed miRNAs in spermatog...Continue Reading

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Citations

Dec 5, 2019·International Journal of Molecular Sciences·Silvia Lorente-CebriánHeriberto Castro
Nov 14, 2020·Biology of Reproduction·Robert NiayaleFredrick Adzitey
Apr 23, 2021·Interdisciplinary Sciences, Computational Life Sciences·Yajun LiuXinhong Hei
Oct 26, 2021·Reproduction in Domestic Animals = Zuchthygiene·Jiabo WangXiaolin Luo

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