Comparative fitness assessment of Anopheles stephensi transgenic lines receptive to site-specific integration.

Insect Molecular Biology
D A AmenyaAnthony A James

Abstract

Genetically modified mosquitoes that are unable to transmit pathogens offer opportunities for controlling vector-borne diseases such as malaria and dengue. Site-specific gene recombination technologies are advantageous in the development of these insects because antipathogen effector genes can be inserted at integration sites in the genome that cause the least alteration in mosquito fitness. Here we describe Anopheles stephensi transgenic lines containing phi C31 attP'docking' sites linked to a fluorescent marker gene. Chromosomal insertion sites were determined and life-table parameters were assessed for transgenic mosquitoes of each line. No significant differences in fitness between the transgenic and nontransgenic mosquitoes were detected in this study. These transgenic lines are suitable for future site-specific integrations of antiparasite transgenes into the attP sites.

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Citations

Jun 13, 2012·Proceedings of the National Academy of Sciences of the United States of America·Alison T IsaacsAnthony A James
Apr 16, 2013·Proceedings of the National Academy of Sciences of the United States of America·Rebeca Carballar-LejarazúAnthony A James
Aug 31, 2011·Journal of Tropical Medicine·Chris M CirimotichGeorge Dimopoulos
Dec 14, 2011·Annual Review of Entomology·Malcolm J Fraser
Nov 26, 2015·Proceedings of the National Academy of Sciences of the United States of America·Valentino M GantzAnthony A James
Mar 17, 2015·Proceedings of the National Academy of Sciences of the United States of America·Sanjay BasuZach N Adelman
Nov 25, 2014·Genome Biology·Paolo GabrieliFlaminia Catteruccia
Oct 15, 2011·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·K Karunamoorthi
Jun 18, 2018·PM & R : the Journal of Injury, Function, and Rehabilitation·Roy La ToucheAlba Paris-Alemany
Jan 1, 2015·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Anam J ArikMichael A Riehle
Sep 10, 2020·Annual Review of Microbiology·E P CaragataG Dimopoulos
Dec 26, 2018·Parasites & Vectors·Irina Häcker, Marc F Schetelig
Jan 9, 2021·Parasites & Vectors·Phillip ShultsCorey Brelsfoard
Mar 26, 2021·BioMed Research International·Hewawasam Patuwatha Badathuruge Kalindu Dulanja RamyasomaRanil Samantha Dassanayake

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