PMID: 7370388Apr 1, 1980Paper

Comparative fluorescence properties of bovine, goat, human and guinea pig alpha lactalbumin. Characterization of the environments of individual tryptophan residues in partially folded conformers

Biophysical Chemistry
P B Sommers, M J Kronman

Abstract

alpha Lactalbumin exists as a partially folded conformer (U form) at acid pH. A second partially folded conformer (H form) is formed above 60 degrees. Comparison of the changes in tryptophan fluorescence which occur on forming U and H for the bovine, goat, human and guinea pig proteins, as well as analysis of fluorescence properties for the bovine protein and an N bromo succinimide derivative of this protein, have made it possible to determine which tryptophan residues give rise to such changes in fluorescence, and to draw a distinction between the molecular structure of the U and H forms of the protein. Trp 28 and 109 in the native state transfer their excitation energy to trp 63 whose fluorescence is quenched by a pair of vicinal disulfide bridges. This process persists in the U form of the protein, but is absent in the H conformer. Most of the change in fluorescence seen in the N in equilibrium with U conversion is due to increase in yield of trp 28, while the changes in fluorescence occurring on formation of the H form are due to exposure of trp 63 and elimination of its quenching and/or excited state transfer from 28 to 109.

References

Feb 1, 1978·Journal of Biochemistry·K TakaseS Arima
Feb 29, 1972·Biochimica Et Biophysica Acta·A O BarelJ Léonis
May 1, 1973·Biochemical and Biophysical Research Communications·P B SommersK Brew
May 1, 1972·European Journal of Biochemistry·J B Findlay, K Brew
Dec 1, 1969·The Journal of Physical Chemistry·R F Steiner, E P Kirby
Apr 19, 1966·Biochemical and Biophysical Research Communications·S S Lehrer, G D Fasman

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Citations

Jan 1, 1989·Critical Reviews in Biochemistry and Molecular Biology·M J Kronman
Mar 1, 1996·International Journal of Peptide and Protein Research·N Tanaka, S Kunugi
Dec 14, 1999·Protein Science : a Publication of the Protein Society·P Polverino de LauretoA Fontana
Jan 1, 1985·Radiation and Environmental Biophysics·A M Edwards, E Silva
Jul 1, 1996·Protein Science : a Publication of the Protein Society·K M CawthernL J Berliner
Oct 7, 1998·Protein Science : a Publication of the Protein Society·S Kim, J Baum
Oct 8, 1999·Journal of Molecular Biology·M MizuguchiK Nitta
Aug 14, 1980·Biochemical and Biophysical Research Communications·Y HiraokaN Murai
Apr 15, 1982·Archives of Biochemistry and Biophysics·P M Mulqueen, M J Kronman
Mar 15, 1984·Biochemical and Biophysical Research Communications·I HanssensF Van Cauwelaert

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