Aug 15, 2009

Comparative gene expression profiles between heterotic and non-heterotic hybrids of tetraploid Medicago sativa

BMC Plant Biology
Xuehui LiE Charles Brummer


Heterosis, the superior performance of hybrids relative to parents, has clear agricultural value, but its genetic control is unknown. Our objective was to test the hypotheses that hybrids expressing heterosis for biomass yield would show more gene expression levels that were different from midparental values and outside the range of parental values than hybrids that do not exhibit heterosis. We tested these hypotheses in three Medicago sativa (alfalfa) genotypes and their three hybrids, two of which expressed heterosis for biomass yield and a third that did not, using Affymetrix M. truncatula GeneChip arrays. Alfalfa hybridized to approximately 47% of the M. truncatula probe sets. Probe set signal intensities were analyzed using MicroArray Suite v.5.0 (MAS) and robust multi-array average (RMA) algorithms. Based on MAS analysis, the two heterotic hybrids performed similarly, with about 27% of genes showing differential expression among the parents and their hybrid compared to 12.5% for the non-heterotic hybrid. At a false discovery rate of 0.15, 4.7% of differentially expressed genes in hybrids (approximately 300 genes) showed nonadditive expression compared to only 0.5% (16 genes) in the non-heterotic hybrid. Of the nonadditive...Continue Reading

Mentioned in this Paper

Real-Time Polymerase Chain Reaction
Microarray Analysis
Stenopterapion meliloti
Nucleic Acid Hybridization Procedure
Transcription, Genetic
Inbred Strain
Medicago truncatula
Array-Based Comparative Genomic Hybridization
Chimera Organism

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