Comparative Gene Expression Profiles of Leishmania major and Leishmania infantum Promastigotesa

Türkiye parazitolojii dergisi
Özlem Ulusan BağcıAyşe Caner

Abstract

This study aimed to determine the differences between the gene expression profiles of Leismania major and Leishmania infantum promastigotes through comparative analysis of gene expressions. Cell culture of L. major (MHOM/IL/80) and L. infantum (MHOM/MA/67/ITMAP/263) cell lines was performed. Afterwards, total RNA isolation and cDNA synthesis were performed and fold changes in the expression levels of 30 genes that play a role in metabolic pathways and nucleic acid synthesis and co-expressed in two species were evaluated by reverse transcriptase polymerase chain reaction. Functions of genes were determined using LeishDB and KEGG databases. In this study, profiles of protein-coding 30 genes expressed in L. major and L. infantum promastigotes were evaluated and significant differences were found between the two species (p<0.001). There was a significant fold change in the expression levels of 29% of genes common in the two species. The expression levels of nine genes in L. major were found to be markedly higher than those of L. infantum (fold change >1). These genes include phosphoglycan beta 1.3 galactosyltransferase-like, lathosterol oxidase-like, fatty acid elongase, 3-oxo-5 alpha-steroid 4-dehydrogenase, calpain-like cysteine ...Continue Reading

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