PMID: 9547283Jun 20, 1998Paper

Comparative gene expression profiling by oligonucleotide fingerprinting

Nucleic Acids Research
S Meier-EwertH Lehrach

Abstract

The use of hybridisation of synthetic oligonucleotides to cDNAs under high stringency to characterise gene sequences has been demonstrated by a number of groups. We have used two cDNA libraries of 9 and 12 day mouse embryos (24 133 and 34 783 clones respectively) in a pilot study to characterise expressed genes by hybridisation with 110 hybridisation probes. We have identified 33 369 clusters of cDNA clones, that ranged in representation from 1 to 487 copies (0.7%). 737 were assigned to known rodent genes, and a further 13 845 showed significant homologies. A total of 404 clusters were identified as significantly differentially represented (P < 0.01) between the two cDNA libraries. This study demonstrates the utility of the fingerprinting approach for the generation of comparative gene expression profiles through the analysis of cDNAs derived from different biological materials.

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Citations

Dec 19, 2001·Developmental Dynamics : an Official Publication of the American Association of Anatomists·T DickmeisU Strähle
Sep 12, 2000·Current Hypertension Reports·M Knoblauch, K Lindpaintner
Jun 14, 2000·Current Opinion in Microbiology·G WalterH Lehrach
May 11, 2005·Journal of Computational Biology : a Journal of Computational Molecular Cell Biology·Chaim Linhart, Ron Shamir
May 10, 2005·Bioinformatics·Thorsten HenrichJoachim Wittbrodt
Nov 16, 2002·Nucleic Acids Research·Andreas W MachlIgor Ivanov
Aug 25, 2000·Genome Research·H EickhoffH Lehrach
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Aug 14, 2002·American Journal of Pharmacogenomics : Genomics-related Research in Drug Development and Clinical Practice·K BüssowG Walter
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Dec 11, 2002·The Plant Journal : for Cell and Molecular Biology·Ralf HerwigUwe Radelof
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Jun 30, 2000·Genomics·E HartuvR Shamir
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Feb 11, 2005·Journal of Computational Biology : a Journal of Computational Molecular Cell Biology·Andres FigueroaTao Jiang

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