Comparative gene expression study of the vestibular organ of the Igf1 deficient mouse using whole-transcript arrays

Hearing Research
Lourdes Rodríguez-de la RosaMarta Milo

Abstract

The auditory and vestibular organs form the inner ear and have a common developmental origin. Insulin like growth factor 1 (IGF-1) has a central role in the development of the cochlea and maintenance of hearing. Its deficiency causes sensorineural hearing loss in man and mice. During chicken early development, IGF-1 modulates neurogenesis of the cochleovestibular ganglion but no further studies have been conducted to explore the potential role of IGF-1 in the vestibular system. In this study we have compared the whole transcriptome of the vestibular organ from wild type and Igf1(-/-) mice at different developmental and postnatal times. RNA was prepared from E18.5, P15 and P90 vestibular organs of Igf1(-/-) and Igf1(+/+) mice and the transcriptome analysed in triplicates using Affymetrix(®) Mouse Gene 1.1 ST Array Plates. These plates are whole-transcript arrays that include probes to measure both messenger (mRNA) and long intergenic non-coding RNA transcripts (lincRNA), with a coverage of over 28 thousand coding transcripts and over 7 thousands non-coding transcripts. Given the complexity of the data we used two different methods VSN-RMA and mmBGX to analyse and compare the data. This is to better evaluate the number of false p...Continue Reading

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Citations

Mar 5, 2016·Frontiers in Neuroscience·Vanesa Nieto-EstévezCarlos Vicario-Abejón
Jan 10, 2018·Frontiers in Aging Neuroscience·Lourdes Rodríguez-de la RosaIsabel Varela-Nieto
Apr 6, 2019·Journal of Neuroscience Research·Kareem O TawfikRick A Friedman
Jun 13, 2017·Frontiers in Cell and Developmental Biology·Marta MagariñosIsabel Varela-Nieto
Apr 13, 2019·Scientific Reports·Alejandro GibajaMarta Magariños
Jul 3, 2021·International Journal of Molecular Sciences·Anna GhilardiLuca Del Giacco

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