Comparative gene transfer between cationic and thiourea lipoplexes

The Journal of Gene Medicine
Marie BretonNathalie Mignet

Abstract

We have previously developed lipopolythiourea lipids as neutral DNA condensing agents for systemic gene delivery. Optimization of the lipopolythiourea structure led to efficient transfecting agents. To further evaluate these lipids, we investigated the internalization process of the thiourea lipoplexes and their intracellular mechanism of transfection versus that of cationic lipoplexes. The MTT test was used for cytotoxicity assessment. Transfection efficiency was determined by luciferase read-out. Permeation to propidium iodide and enhanced green fluorescent protein was evaluated by flow cytometry. Kinetics of internalization and DNA release were monitored by confocal microscopy with labelled DNA. Endocytosis inhibitors were used to study the mechanisms of lipoplex internalization. Although thiourea/DNA complexes exhibit an almost similar level of transfection compared to that of cationic complexes, the thiourea lipoplexes were shown to be six-fold less internalized. Complexes were able to permeabilize the cytoplasmic membrane to 30 kDa molecules. Finally, DNA was shown to be released in less than 10 min in the cellular cytoplasm versus 30 min for cationic lipoplexes. Despite a weaker internalization compared to cationic lipid...Continue Reading

References

Sep 25, 1998·The Journal of Biological Chemistry·L C MounkesR J Debs
Nov 18, 2004·Bioconjugate Chemistry·Isabelle TranchantJean Herscovici
Nov 27, 2004·Molecular Therapy : the Journal of the American Society of Gene Therapy·Ikuhiko NakaseShiroh Futaki
May 19, 2005·Bioconjugate Chemistry·B ThompsonM Bessodes
Jun 21, 2005·Molecular Therapy : the Journal of the American Society of Gene Therapy·Joanna RejmanMassimo Conese
Dec 6, 2005·The Journal of Biological Chemistry·Ikramy A KhalilHideyoshi Harashima
Feb 14, 2006·Angewandte Chemie·Ralf BausingerAndreas Zumbusch
Mar 2, 2006·Pharmacological Reviews·Ikramy A KhalilHideyoshi Harashima
Aug 18, 2006·Journal of Controlled Release : Official Journal of the Controlled Release Society·Luc Wasungu, Dick Hoekstra
Sep 6, 2006·Journal of Liposome Research·Joanna RejmanDick Hoekstra
Sep 21, 2006·Bioconjugate Chemistry·Jeanne LeblondJean Herscovici
Jan 20, 2007·Biochemical Society Transactions·D HoekstraI Zuhorn
Mar 22, 2007·Bioconjugate Chemistry·Jeanne LeblondJean Herscovici
Jul 7, 2007·Stem Cells·E Scott SwensonDiane S Krause
Dec 8, 2007·Bioconjugate Chemistry·Jeanne LeblondJean Herscovici

❮ Previous
Next ❯

Citations

Dec 3, 2014·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Johanne SeguinNathalie Mignet
Nov 3, 2010·Chemical Society Reviews·Carmen Ortiz MelletJuan M Benito
Jun 7, 2012·Bioconjugate Chemistry·Nathalie SymensJoanna Rejman
Oct 24, 2011·Pharmaceuticals·Marie BretonNathalie Mignet
Jun 9, 2010·The Journal of Gene Medicine·Nathalie MignetDaniel Scherman
May 8, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Lucie ValeroKarine Andrieux
Aug 30, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Marie BretonNathalie Mignet

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
P L FelgnerM Danielsen
Proceedings of the National Academy of Sciences of the United States of America
J P BehrJ Perez-Mutul
Advanced Drug Delivery Reviews
Christine DufèsAndreas G Schätzlein
Molecular Therapy : the Journal of the American Society of Gene Therapy
Tuomas T Rissanen, Seppo Ylä-Herttuala
© 2022 Meta ULC. All rights reserved