Comparative genomic analysis of Acinetobacter spp. plasmids originating from clinical settings and environmental habitats

Scientific Reports
Ileana P SaltoMariano Pistorio

Abstract

Bacteria belonging to the genus Acinetobacter have become of clinical importance over the last decade due to the development of a multi-resistant phenotype and their ability to survive under multiple environmental conditions. The development of these traits among Acinetobacter strains occurs frequently as a result of plasmid-mediated horizontal gene transfer. In this work, plasmids from nosocomial and environmental Acinetobacter spp. collections were separately sequenced and characterized. Assembly of the sequenced data resulted in 19 complete replicons in the nosocomial collection and 77 plasmid contigs in the environmental collection. Comparative genomic analysis showed that many of them had conserved backbones. Plasmid coding sequences corresponding to plasmid specific functions were bioinformatically and functionally analyzed. Replication initiation protein analysis revealed the predominance of the Rep_3 superfamily. The phylogenetic tree constructed from all Acinetobacter Rep_3 superfamily plasmids showed 16 intermingled clades originating from nosocomial and environmental habitats. Phylogenetic analysis of relaxase proteins revealed the presence of a new sub-clade named MOBQAci, composed exclusively of Acinetobacter relax...Continue Reading

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Citations

Apr 8, 2020·Frontiers in Microbiology·Verónica Elizabeth ÁlvarezDaniela Centrón
Jul 7, 2020·Frontiers in Microbiology·Abraham D Salgado-CamargoMiguel A Cevallos
Jul 14, 2020·World Journal of Microbiology & Biotechnology·Marco A BrovedanGuillermo D Repizo
Dec 12, 2020·Frontiers in Microbiology·Sofia MindlinMayya Petrova
Jul 3, 2021·Water Research·Nuno F F MoreiraOlga C Nunes
Aug 8, 2021·International Journal of Molecular Sciences·Fernando Sanz-GarcíaSara Hernando-Amado
Nov 4, 2021·Scientific Reports·María Florencia PerezJulián Rafael Dib
Nov 30, 2021·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·James P J HallDavid A Baltrus

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Datasets Mentioned

BETA
PRJEB22129

Methods Mentioned

BETA
electrophoresis
PCR

Software Mentioned

GenDB
Easyfig
ProtTets
gs Assembler
BLASTP
PhyML
GS de novo Assembler
ClustalW
CONSED

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