Comparative Genomic Analysis of Virulence, Antimicrobial Resistance, and Plasmid Profiles of Salmonella Dublin Isolated from Sick Cattle, Retail Beef, and Humans in the United States

Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease
Chih-Hao HsuShaohua Zhao

Abstract

Salmonella enterica serovar Dublin is a host-adapted serotype associated with typhoidal disease in cattle. While rare in humans, it usually causes severe illness, including bacteremia. In the United States, Salmonella Dublin has become one of the most multidrug-resistant (MDR) serotypes. To understand the genetic elements that are associated with virulence and resistance, we sequenced 61 isolates of Salmonella Dublin (49 from sick cattle and 12 from retail beef) using the Illumina MiSeq and closed 5 genomes using the PacBio sequencing platform. Genomic data of eight human isolates were also downloaded from NCBI (National Center for Biotechnology Information) for comparative analysis. Fifteen Salmonella pathogenicity islands (SPIs) and a spv operon (spvRABCD), which encodes important virulence factors, were identified in all 69 (100%) isolates. The 15 SPIs were located on the chromosome of the 5 closed genomes, with each of these isolates also carrying 1 or 2 plasmids with sizes between 36 and 329 kb. Multiple antimicrobial resistance genes (ARGs), including blaCMY-2, blaTEM-1B, aadA12, aph(3')-Ia, aph(3')-Ic, strA, strB, floR, sul1, sul2, and tet(A), along with spv operons were identified on these plasmids. Comprehensive antimi...Continue Reading

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Citations

Oct 4, 2020·Journal of Veterinary Pharmacology and Therapeutics·Patrick F McDermott, James J Davis
Jun 11, 2021·Frontiers in Microbiology·Belén González-SantamarinaHerbert Tomaso

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Methods Mentioned

BETA
PCR

Software Mentioned

BLAST
PlasmidFinder
SNPhylo
Perl scripts
PacBio Hierarchical Genome Assembly Process ( HGAP3 . 0 )
ResFinder

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