Abstract
Comparative genomic hybridization (CGH) is a powerful technique for detecting copy number changes throughout the genome. We describe the development of a versatile image analysis program for CGH studies. Several methods for the production of metaphases which give optimum hybridization signals have also been assessed. CGH analysis was performed on DNA samples from several different and clinically relevant specimens: amniotic fluid cells trisomic for a single chromosome, lymphoblastoid cell lines with abnormalities involving single chromosome bands, malignant cell lines and biopsy material from primary ovarian carcinomas. The results were compared with those derived from G-banding, chromosome painting, and molecular genetic techniques. Our data demonstrate that CGH was able to detect a wide range of quantitative genetic alterations including duplication or deletion of single chromosome bands. CGH analysis also indicated the presence of genetic abnormalities that were not detected by other cytogenetic or molecular approaches. Moreover, our CGH methodology allowed the ready comparison of CGH results from different tumors, a process which greatly facilitated identification of shared genetic changes.
References
Feb 1, 1978·Experimental Cell Research·D SchweizerM Andrle
Apr 1, 1992·Nature Genetics·J R LupskiP I Patel
Oct 30, 1992·Science·A KallioniemiD Pinkel
Mar 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·K AlitaloJ M Bishop
Nov 1, 1993·Genes, Chromosomes & Cancer·A N MohamedS R Wolman
Apr 1, 1994·Genes, Chromosomes & Cancer·R F SuijkerbuijkA Geurts van Kessel
Aug 1, 1994·Genes, Chromosomes & Cancer·O P KallioniemiD Pinkel
Mar 1, 1995·Genes, Chromosomes & Cancer·M BentzP Lichter
Mar 1, 1995·Genes, Chromosomes & Cancer·A KallioniemiF Waldman
Jun 1, 1995·Genes, Chromosomes & Cancer·G MohapatraB G Feuerstein
Jul 1, 1995·Cancer Genetics and Cytogenetics·E NachevaA R Green
Jan 1, 1995·Cytometry·J PiperD Pinkel
Jan 1, 1995·Cytometry·S du ManoirT Cremer
Jan 1, 1995·Cytometry·S du ManoirM Giollant
Jan 1, 1995·Cytometry·C LundsteenT Gerdes
Mar 16, 1995·International Journal of Cancer. Journal International Du Cancer·D H KimB G Feuerstein
Nov 1, 1994·Blood·F A AsimakopoulosA R Green
Mar 15, 1994·Proceedings of the National Academy of Sciences of the United States of America·A KallioniemiF M Waldman
Apr 1, 1994·Cancer Research·T RiedT Cremer
Nov 1, 1993·Human Molecular Genetics·M R SpeicherT Ried
Feb 1, 1993·Human Genetics·S du ManoirT Cremer
Citations
Dec 5, 2000·Cancer Genetics and Cytogenetics·A JacobsenM Kiechle
Jul 21, 2001·Cancer Genetics and Cytogenetics·Y HerishanuA Amiel
May 30, 2002·Cancer Genetics and Cytogenetics·Elida Paula Benquique OjopiSilvia Regina Rogatto
Jun 20, 2002·Cancer Genetics and Cytogenetics·Caterina MatteucciCristina Mecucci
May 18, 1999·Cancer Genetics and Cytogenetics·S M GribbleE P Nacheva
Jun 28, 2001·BJOG : an International Journal of Obstetrics and Gynaecology·A TheinP Soothill
Mar 22, 2001·Clinical Genetics·M A RigolaC Fuster
Sep 8, 2004·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Christine PirkerWalter Berger
Dec 18, 2001·American Journal of Medical Genetics·M A RigolaC Fuster
Mar 13, 2001·Leukemia·A ViardotB Schlegelberger
Jul 9, 1999·The Journal of Pathology·L A James
Oct 31, 2002·American Journal of Medical Genetics·Nancy Wang
Jul 21, 2001·European Journal of Human Genetics : EJHG·B FritzH Rehder
Feb 7, 2002·Blood·Stefan JoosThomas F E Barth
Dec 3, 2002·Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B·Judith W M JeukenPieter Wesseling
Oct 20, 2000·American Journal of Medical Genetics·B FritzC Fonatsch