Comparative genomic hybridization: a comparison with molecular and cytogenetic analysis

Cancer Genetics and Cytogenetics
E NachevaA R Green

Abstract

Comparative genomic hybridization (CGH) is a powerful technique for detecting copy number changes throughout the genome. We describe the development of a versatile image analysis program for CGH studies. Several methods for the production of metaphases which give optimum hybridization signals have also been assessed. CGH analysis was performed on DNA samples from several different and clinically relevant specimens: amniotic fluid cells trisomic for a single chromosome, lymphoblastoid cell lines with abnormalities involving single chromosome bands, malignant cell lines and biopsy material from primary ovarian carcinomas. The results were compared with those derived from G-banding, chromosome painting, and molecular genetic techniques. Our data demonstrate that CGH was able to detect a wide range of quantitative genetic alterations including duplication or deletion of single chromosome bands. CGH analysis also indicated the presence of genetic abnormalities that were not detected by other cytogenetic or molecular approaches. Moreover, our CGH methodology allowed the ready comparison of CGH results from different tumors, a process which greatly facilitated identification of shared genetic changes.

References

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Citations

Jul 21, 2001·Cancer Genetics and Cytogenetics·Y HerishanuA Amiel
May 30, 2002·Cancer Genetics and Cytogenetics·Elida Paula Benquique OjopiSilvia Regina Rogatto
Jun 28, 2001·BJOG : an International Journal of Obstetrics and Gynaecology·A TheinP Soothill
Sep 8, 2004·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Christine PirkerWalter Berger
Dec 18, 2001·American Journal of Medical Genetics·M A RigolaC Fuster
Jul 9, 1999·The Journal of Pathology·L A James
Oct 31, 2002·American Journal of Medical Genetics·Nancy Wang
Dec 3, 2002·Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B·Judith W M JeukenPieter Wesseling

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