Comparative Genomic, MicroRNA, and Tissue Analyses Reveal Subtle Differences between Non-Diabetic and Diabetic Foot Skin

PloS One
Horacio A RamirezMarjana Tomic-Canic

Abstract

Diabetes Mellitus (DM) is a chronic, severe disease rapidly increasing in incidence and prevalence and is associated with numerous complications. Patients with DM are at high risk of developing diabetic foot ulcers (DFU) that often lead to lower limb amputations, long term disability, and a shortened lifespan. Despite this, the effects of DM on human foot skin biology are largely unknown. Thus, the focus of this study was to determine whether DM changes foot skin biology predisposing it for healing impairment and development of DFU. Foot skin samples were collected from 20 patients receiving corrective foot surgery and, using a combination of multiple molecular and cellular approaches, we performed comparative analyses of non-ulcerated non-neuropathic diabetic foot skin (DFS) and healthy non-diabetic foot skin (NFS). MicroRNA (miR) profiling of laser captured epidermis and primary dermal fibroblasts from both DFS and NFS samples identified 5 miRs de-regulated in the epidermis of DFS though none reached statistical significance. MiR-31-5p and miR-31-3p were most profoundly induced. Although none were significantly regulated in diabetic fibroblasts, miR-29c-3p showed a trend of up-regulation, which was confirmed by qPCR in a pros...Continue Reading

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Dec 24, 2017·The Journal of Investigative Dermatology·Horacio A RamirezMarjana Tomic-Canic

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Datasets Mentioned

BETA
GSE68186

Methods Mentioned

BETA
amputations
laser capture microdissection
PCR
biopsies
light microscopy
biopsy

Software Mentioned

ImageJ
Exiqon GenEX
nSolver2
Expression Console
Transcriptome Analysis Console ( TAC )
Elements BR3
Affymetrix Command Console Software ( AGCC
Prism
NIS

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