Comparative genomics analysis of pKF3-94 in Klebsiella pneumoniae reveals plasmid compatibility and horizontal gene transfer

Frontiers in Microbiology
Jianchao YingJunwan Lu

Abstract

In order to get insights into plasmid evolution and the dissemination of multidrug resistance, we performed extensive comparative genomics analyses of the Klebsiella pneumoniae plasmid pKF3-94 and some of its related plasmids. pKF3-94 is one of three plasmids isolated from the K. pneumoniae strain KF3. Of the 144 putative genes it harbors, 69 can be functionally assigned to be involved in transfer conjugation, transfer leading, antimicrobial resistance, transposon function, and plasmid replication. Comparison of plasmid replicon sequence types revealed that pKF3-94 carries two replicons that are distinct from those carried on the two sibling K. pneumonia plasmids pKF3-70 and pKF3-140, thereby allowing pKF3-94 to coexist with these latter plasmids in the same host cell. Comparative genomics analyses further showed that pKF3-94 is more similar to plasmids pK1HV and pC15-k, which were isolated from different K. pneumonia strains, than to pKF3-70 and pKF3-140. Interestingly, pK1HV contains a unique 49 kb region rich in mobile genetic elements and drug resistance genes, while pKF3-94 and pC15-k share a 15 kb homology region partitioned into a region rich in drug resistance genes and one containing a replicon. It is conceivable, ther...Continue Reading

References

Jun 15, 1999·Plasmid·N P ManwaringN Firth
Dec 18, 2001·Journal of Molecular Biology·M RemmE L Sonnhammer
Jun 5, 2003·Molecular Biology and Evolution·Ravi JainJames A Lake
Dec 14, 2004·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Sudhindra R GadagkarSudhir Kumar
Jun 28, 2005·Nucleic Acids Research·Gary H Van DomselaarDavid S Wishart
Dec 31, 2005·Nucleic Acids Research·P SiguierM Chandler
Mar 10, 2009·Methods in Molecular Biology·Martin Iain BahlSøren J Sørensen
Mar 10, 2009·Methods in Molecular Biology·Patricia A Sobecky, Jonna M Coombs
Mar 2, 2010·Applied Microbiology and Biotechnology·Matthias Brigulla, Wilfried Wackernagel
Apr 28, 2010·Antimicrobial Agents and Chemotherapy·Zhiyong ZongJonathan R Iredell
Oct 12, 2010·The Journal of Antimicrobial Chemotherapy·Laura VillaAlessandra Carattoli
Mar 12, 2011·The Journal of Antimicrobial Chemotherapy·Jannine K BaileyRuth M Hall
Dec 12, 2012·Antimicrobial Agents and Chemotherapy·Monika DolejskaAlessandra Carattoli
Jan 19, 2013·Molecular Biology and Evolution·Kazutaka Katoh, Daron M Standley

❮ Previous
Next ❯

Citations

Sep 7, 2017·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Zhenbo XuMark E Shirtliff
Apr 1, 2020·Journal of Medical Microbiology·Lijiang ChenTieli Zhou
Jun 15, 2021·Genome Biology·Askarbek OrakovPeer Bork

❮ Previous
Next ❯

Datasets Mentioned

BETA
BAA-2146

Methods Mentioned

BETA
RepA
phosphotransferase
DNA shearing
electrophoresis

Software Mentioned

MAFFT
Phred
modelgenerator
Phrap
BASys
ISFinder
Glimmer3
traT
Python
BLASTp

Related Concepts

Related Feeds

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.