Comparative Genomics and Identification of an Enterotoxin-Bearing Pathogenicity Island, SEPI-1/SECI-1, in Staphylococcus epidermidis Pathogenic Strains

Toxins
Xavier ArgemiGilles Prévost

Abstract

Staphylococcus epidermidis is a leading cause of nosocomial infections, majorly resistant to beta-lactam antibiotics, and may transfer several mobile genetic elements among the members of its own species, as well as to Staphylococcus aureus; however, a genetic exchange from S. aureus to S. epidermidis remains controversial. We recently identified two pathogenic clinical strains of S. epidermidis that produce a staphylococcal enterotoxin C3-like (SEC) similar to that by S. aureus pathogenicity islands. This study aimed to determine the genetic environment of the SEC-coding sequence and to identify the mobile genetic elements. Whole-genome sequencing and annotation of the S. epidermidis strains were performed using Illumina technology and a bioinformatics pipeline for assembly, which provided evidence that the SEC-coding sequences were located in a composite pathogenicity island that was previously described in the S. epidermidis strain FRI909, called SePI-1/SeCI-1, with 83.8-89.7% nucleotide similarity. Various other plasmids were identified, particularly p_3_95 and p_4_95, which carry antibiotic resistance genes (hsrA and dfrG, respectively), and share homologies with SAP085A and pUSA04-2-SUR11, two plasmids described in S. aur...Continue Reading

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Citations

Nov 27, 2019·Genome Biology and Evolution·Sylwia BanaszkiewiczJacek Bania
Mar 14, 2019·International Journal of Molecular Sciences·Xavier ArgemiGilles Prévost

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Datasets Mentioned

BETA
GQ900437.1
CP022247.1
HG813242
CP009046
CP000029.1
CP018842.1
CP020463.1

Methods Mentioned

BETA
electrophoresis

Software Mentioned

PHage Search Tool
WebMGA
IslandViewer
DIMOB
EMBOSS Needle
IslandPath
SPAdes
BLAST
Easyfig
Clustal Omega

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