Comparative genomics and transcriptomics of 4 Paragonimus species provide insights into lung fluke parasitism and pathogenesis.

GigaScience
Bruce A RosaMakedonka Mitreva

Abstract

Paragonimus spp. (lung flukes) are among the most injurious foodborne helminths, infecting ∼23 million people and subjecting ∼292 million to infection risk. Paragonimiasis is acquired from infected undercooked crustaceans and primarily affects the lungs but often causes lesions elsewhere including the brain. The disease is easily mistaken for tuberculosis owing to similar pulmonary symptoms, and accordingly, diagnostics are in demand. We assembled, annotated, and compared draft genomes of 4 prevalent and distinct Paragonimus species: Paragonimus miyazakii, Paragonimus westermani, Paragonimus kellicotti, and Paragonimus heterotremus. Genomes ranged from 697 to 923 Mb, included 12,072-12,853 genes, and were 71.6-90.1% complete according to BUSCO. Orthologous group analysis spanning 21 species (lung, liver, and blood flukes, additional platyhelminths, and hosts) provided insights into lung fluke biology. We identified 256 lung fluke-specific and conserved orthologous groups with consistent transcriptional adult-stage Paragonimus expression profiles and enriched for iron acquisition, immune modulation, and other parasite functions. Previously identified Paragonimus diagnostic antigens were matched to genes, providing an opportunity...Continue Reading

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Citations

Jan 9, 2021·Parasitology Research·Kurt C CurtisPeter U Fischer
Mar 23, 2021·Tropical Parasitology·David Blair
Jun 15, 2021·Frontiers in Cellular and Infection Microbiology·Santiago FontenlaJose F Tort

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Datasets Mentioned

BETA
PRJNA219632
152
SRX1100074
PRJNA245325
GM097435

Methods Mentioned

BETA
whole-genome shotgun
RNA-seq
enzyme-linked immunosorbent assay

Software Mentioned

AUGUSTUS
Ensembl
PacBio
PosiGene
RepeatModeler
PANNZER2
GhostKOALA
RepeatMasker
LG
OrthoFinder

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