Comparative Genomics Determines Strain-Dependent Secondary Metabolite Production in Streptomyces venezuelae Strains.

Biomolecules
Woori KimByung-Kwan Cho

Abstract

Streptomyces venezuelae is well known to produce various secondary metabolites, including chloramphenicol, jadomycin, and pikromycin. Although many strains have been classified as S. venezuelae species, only a limited number of strains have been explored extensively for their genomic contents. Moreover, genomic differences and diversity in secondary metabolite production between the strains have never been compared. Here, we report complete genome sequences of three S. venezuelae strains (ATCC 10712, ATCC 10595, and ATCC 21113) harboring chloramphenicol and jadomycin biosynthetic gene clusters (BGC). With these high-quality genome sequences, we revealed that the three strains share more than 85% of total genes and most of the secondary metabolite biosynthetic gene clusters (smBGC). Despite such conservation, the strains produced different amounts of chloramphenicol and jadomycin, indicating differential regulation of secondary metabolite production at the strain level. Interestingly, antagonistic production of chloramphenicol and jadomycin was observed in these strains. Through comparison of the chloramphenicol and jadomycin BGCs among the three strains, we found sequence variations in many genes, the non-coding RNA coding regi...Continue Reading

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Citations

Jan 27, 2021·Biomolecules·Eung-Soo Kim
Apr 12, 2021·Briefings in Bioinformatics·Anqiang JiaYi Wang
Jun 9, 2021·Journal of Industrial Microbiology & Biotechnology·Juan Pablo Gomez-EscribanoMervyn J Bibb

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Datasets Mentioned

BETA
CP029197
CP029196
CP029195
FR845719

Methods Mentioned

BETA
PCR

Software Mentioned

PYANI
Protein BLAST
Perl
antiSMASH
OrthoVenn2
ANIm
SAM
MUMmer
CLC workbench
EasyFig

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