DOI: 10.1101/519546Jan 17, 2019Paper

Comparative genomics: Dominant coral bacterium Endozoicomonas acroporae metabolizes Dimethylsulfoniopropionate (DMSP)

BioRxiv : the Preprint Server for Biology
Kshitij TandonSen-Lin Tang

Abstract

Dominant coral-associated Endozoicomonas bacteria species are hypothesized to play a role in the coral-sulfur cycle by metabolizing Dimethylsulfoniopropionate (DMSP) into Dimethylsulfide (DMS); however, no sequenced genome to date harbors genes for this process. In this study, we assembled high-quality (>95% complete) genomes of strains of a recently added species Endozoicomonas acroporae (Acr-14T, Acr-1 and Acr-5) isolated from the coral Acropora muricata and performed comparative genomic analysis on genus Endozoicomonas . We identified the first DMSP CoA-transferase/lyase-a dddD gene homolog found in all E. acroporae strains and functionally characterized bacteria capable of metabolizing DMSP into DMS via the DddD cleavage pathway using RT-qPCR and gas chromatography (GC). Furthermore, we demonstrated that E. acroporae strains can use DMSP as the sole carbon source and have genes arranged in an operon like manner to link DMSP metabolism to the central carbon cycle. This study confirms the role of Endozoicomonas in the coral sulfur cycle.

Related Concepts

Carbon
Gas Chromatography
Genes
Genome
Operon
Sulfur
Dimethylpropiothetin
Dimethylsulfide - acceptor oxidoreductase
Proteolysis
Comparative Genomic Analysis

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