Comparative genomics identifies new alpha class genes within the avian glutathione S-transferase gene cluster

Gene
Ji Eun KimR A Coulombe

Abstract

Glutathione S-transferases (GSTs: EC2.5.1.18) are a superfamily of multifunctional dimeric enzymes that catalyze the conjugation of glutathione (GSH) to electrophilic chemicals. In most animals and in humans, GSTs are the principal enzymes responsible for detoxifying the mycotoxin aflatoxin B(1) (AFB(1)) and GST dysfunction is a known risk factor for susceptibility towards AFB(1). Turkeys are one of the most susceptible animals known to AFB(1), which is a common contaminant of poultry feeds. The extreme susceptibility of turkeys is associated with hepatic GSTs unable to detoxify the highly reactive and electrophilic metabolite exo-AFB(1)-8,9-epoxide (AFBO). In this study, comparative genomic approaches were used to amplify and identify the alpha-class tGST genes (tGSTA1.1, tGSTA1.2, tGSTA1.3, tGSTA2, tGSTA3 and tGSTA4) from turkey liver. The conserved GST domains and four alpha-class signature motifs in turkey GSTs (with the exception of tGSTA1.1 which lacked one motif) confirm the presence of hepatic alpha-class GSTs in the turkey. Four signature motifs and conserved residues found in alpha-class tGSTs are (1) xMExxxWLLAAAGVE, (2) YGKDxKERAxIDMYVxG, (3) PVxEKVLKxHGxxxL and (4) PxIKKFLXPGSxxKPxxx. A BAC clone containing the alp...Continue Reading

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Citations

Aug 31, 2011·Toxicological Sciences : an Official Journal of the Society of Toxicology·Ji Eun KimRoger A Coulombe
May 29, 2013·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Brett R BundersonRoger A Coulombe
May 28, 2011·Toxicology and Applied Pharmacology·Sumit Rawal, Roger A Coulombe
May 14, 2010·Research in Veterinary Science·Sumit RawalRoger Coulombe
Jan 13, 2018·Toxicology Mechanisms and Methods·He WangXiuying Zhang

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