Comparative genomics of Cluster O mycobacteriophages

PloS One
Steven G CresawnGraham F Hatfull

Abstract

Mycobacteriophages--viruses of mycobacterial hosts--are genetically diverse but morphologically are all classified in the Caudovirales with double-stranded DNA and tails. We describe here a group of five closely related mycobacteriophages--Corndog, Catdawg, Dylan, Firecracker, and YungJamal--designated as Cluster O with long flexible tails but with unusual prolate capsids. Proteomic analysis of phage Corndog particles, Catdawg particles, and Corndog-infected cells confirms expression of half of the predicted gene products and indicates a non-canonical mechanism for translation of the Corndog tape measure protein. Bioinformatic analysis identifies 8-9 strongly predicted SigA promoters and all five Cluster O genomes contain more than 30 copies of a 17 bp repeat sequence with dyad symmetry located throughout the genomes. Comparison of the Cluster O phages provides insights into phage genome evolution including the processes of gene flux by horizontal genetic exchange.

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Citations

Jun 22, 2016·The ISME Journal·François EnaultMarie-Agnès Petit
Mar 30, 2019·Microbiology Resource Announcements·Travis MillerDustin Edwards
Oct 16, 2018·MSystems·Gareth TrublVirginia I Rich
Jun 20, 2020·PloS One·Deborah Jacobs-SeraGraham F Hatfull
May 26, 2018·Genome Announcements·Casey JangArturo Diaz
Apr 6, 2016·Journal of Microbiology & Biology Education·Scott FreemanFerric C Fang

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Datasets Mentioned

BETA
AY129335
KJ829260
70214

Methods Mentioned

BETA
PCR

Software Mentioned

Scaffold
Scaffold4
ClustalW
BLASTP
DNAMaster
Catdawg
BLASTN
TMHMM
Corndog
HHPred

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