Comparative Genomics of Plasmid-Bearing Staphylococcus aureus Strains Isolated From Various Retail Meats

Frontiers in Microbiology
Anand B KarkiMohamed K Fakhr

Abstract

Food poisoning due to the consumption of Staphylococcus aureus contaminated food is a major health problem worldwide. In this study, we sequenced the genomes of ten plasmid-bearing S. aureus strains isolated from retail beef, chicken, turkey, and pork. The chromosomes of the strains varied in size from 2,654,842 to 2,807,514 bp, and a total of 25 plasmids were identified ranging from 1.4 to 118 kb. Comparative genomic analysis revealed similarities between strains isolated from the same retail meat source, indicating an origin-specific genomic composition. Genes known to modulate attachment, invasion, and toxin production were identified in the 10 genomes. Strains from retail chicken resembled human clinical isolates with respect to virulence factors and genomic islands, and retail turkey and pork isolates shared similarity with S. aureus from livestock. Most chromosomes contained antimicrobial resistance, heavy metal resistance, and stress response genes, and several plasmids contained genes involved in antimicrobial resistance and virulence. In conclusion, the genomes of S. aureus strains isolated from retail meats showed an origin-specific composition and contained virulence and antimicrobial resistance genes similar to thos...Continue Reading

References

Jun 5, 2002·Lancet·Tadashi BabaKeiichi Hiramatsu
Mar 23, 2004·Nucleic Acids Research·Robert C Edgar
Mar 31, 2009·Journal of Bacteriology·Christiane GoerkeChristiane Wolz
Jun 25, 2009·BMC Evolutionary Biology·Denice C Bay, Raymond J Turner
Oct 9, 2009·Journal of Clinical Microbiology·Vasanthakumari NeelaEhsanollah Ghaznavi Rad
Nov 4, 2009·Proceedings of the National Academy of Sciences of the United States of America·Bethan V LowderJ Ross Fitzgerald
Oct 12, 2010·Vector Borne and Zoonotic Diseases·Tara C Smith, Nicole Pearson
Apr 19, 2011·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Andrew E WatersLance B Price
Jul 19, 2011·Emerging Infectious Diseases·Craig W Hedberg
Jul 1, 2010·Toxins·María Ángeles ArgudínMaría Rosario Rodicio
Nov 19, 2011·FEMS Microbiology Reviews·Jacques-Antoine HennekinneSylviane Dragacci
Dec 14, 2011·PloS One·Anne-Sophie Valentin-DomelierUNKNOWN Bloodstream Infection Study Group of the Réseau des Hygiénistes du Centre (RHC)
Jan 13, 2012·Journal of Clinical Microbiology·Mette V LarsenOle Lund
Jul 12, 2012·The Journal of Antimicrobial Chemotherapy·Ea ZankariMette Voldby Larsen
Aug 2, 2012·The EMBO Journal·Alexander J LaarmanSuzan H M Rooijakkers
Oct 10, 2012·Current Opinion in Microbiology·José R MediavillaBarry N Kreiswirth
Apr 10, 2013·The Open Microbiology Journal·Sofia Santos CostaIsabel Couto
May 11, 2013·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Guoqing Xia, Christiane Wolz
Nov 15, 2013·Nucleic Acids Research·Alice R WattamBruno W Sobral
Dec 3, 2013·Nucleic Acids Research·Ross OverbeekRick Stevens
Feb 8, 2014·PloS One·Pimlapas LeekitcharoenphonFrank M Aarestrup
Feb 28, 2014·Journal of Clinical Microbiology·Katrine Grimstrup JoensenFrank M Aarestrup
May 8, 2014·BioMed Research International·Jhalka KadariyaDipendra Thapaliya
Oct 10, 2014·Journal of Clinical Microbiology·Mette Damkjær BartelsHenrik Westh
May 29, 2015·Clinical Microbiology Reviews·Steven Y C TongVance G Fowler
Nov 27, 2015·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·Joël MossongPierre Weicherding
May 5, 2016·Nucleic Acids Research·David ArndtDavid S Wishart
Jun 12, 2016·Proceedings of the National Academy of Sciences of the United States of America·Emanuele BosiBernhard Ø Palsson
Jul 29, 2016·Bioinformatics·Dmitry AntipovPavel A Pevzner

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Citations

Nov 20, 2021·Frontiers in Microbiology·Stéphanie NouwsSigrid C J De Keersmaecker

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Methods Mentioned

BETA
PCR
Assay

Software Mentioned

Island viewer
CLC workbench
BLASTn
PHASTER
BLASTp
PATRIC
MLST
Blast
Seed Viewer ( RAST )
VFanalyzer

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