Comparative genomics of Roseobacter clade bacteria isolated from the accessory nidamental gland of Euprymna scolopes

Frontiers in Microbiology
Andrew J CollinsSpencer V Nyholm

Abstract

The accessory nidamental gland (ANG) of the female Hawaiian bobtail squid, Euprymna scolopes, houses a consortium of bacteria including members of the Flavobacteriales, Rhizobiales, and Verrucomicrobia but is dominated by members of the Roseobacter clade (Rhodobacterales) within the Alphaproteobacteria. These bacteria are deposited into the jelly coat of the squid's eggs, however, the function of the ANG and its bacterial symbionts has yet to be elucidated. In order to gain insight into this consortium and its potential role in host reproduction, we cultured 12 Rhodobacterales isolates from ANGs of sexually mature female squid and sequenced their genomes with Illumina sequencing technology. For taxonomic analyses, the ribosomal proteins of 79 genomes representing both roseobacters and non-roseobacters along with a separate MLSA analysis of 33 housekeeping genes from Roseobacter organisms placed all 12 isolates from the ANG within two groups of a single Roseobacter clade. Average nucelotide identity analysis suggests the ANG isolates represent three genera (Leisingera, Ruegeria, and Tateyamaria) comprised of seven putative species groups. All but one of the isolates contains a predicted Type VI secretion system, which has been s...Continue Reading

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Citations

Feb 1, 2017·Environmental Microbiology·Ying SunHaiwei Luo
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Aug 11, 2020·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Spencer V Nyholm
Jun 6, 2021·Nature Reviews. Microbiology·Spencer V Nyholm, Margaret J McFall-Ngai

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Datasets Mentioned

BETA
AFCF00000000

Methods Mentioned

BETA
Diamond filtration
biosensor
glycosylase

Software Mentioned

CLC Genomic Workbench ( CLC )
seqret
MUSCLE
BAGEL
JSpecies1
AntiSMASH
RAST
BActeriocin Genome mining
EMBOSS
In

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